OBJECTIVE: We investigated the frequencies of the functionally important variants of the CYP2A6 gene in black African populations. METHODS: Using genomic DNA sequencing, polymerase chain reaction (PCR)-restriction fragment length polymorphism and allele-specific PCR, the allele frequencies of CYP2A6 *1A, *1B, *2, *4A, *5, *6, *7, *8, *9, *10 and * 11 among 120 black Africans- including 105 Ghanaians, 12 Nigerians, 2 Ivorians and 1 Ugandan-were determined. RESULTS: The allele frequencies were 80.5% for CYP2A6*1A, 11.9% for CYP2A6*1B, 1.9% for CYP2A6*4A and 5.7% for CYP2A6*9 in the Ghanaian subjects. No subject homozygous for the CYP2A6*4A allele, a whole gene deletion type of polymorphism prevalent among Orientals, was found. Furthermore, CYP2A6 variants such as *2, *5, *6, *7, *8, *10 and *11 were absent in these black African populations. CONCLUSIONS: This study provides, for the first time, the results of the analysis of CYP2A6 allele frequency in black African populations and confirms large ethnic differences in the polymorphic CYP2A6 gene.
OBJECTIVE: We investigated the frequencies of the functionally important variants of the CYP2A6 gene in black African populations. METHODS: Using genomic DNA sequencing, polymerase chain reaction (PCR)-restriction fragment length polymorphism and allele-specific PCR, the allele frequencies of CYP2A6 *1A, *1B, *2, *4A, *5, *6, *7, *8, *9, *10 and * 11 among 120 black Africans- including 105 Ghanaians, 12 Nigerians, 2 Ivorians and 1 Ugandan-were determined. RESULTS: The allele frequencies were 80.5% for CYP2A6*1A, 11.9% for CYP2A6*1B, 1.9% for CYP2A6*4A and 5.7% for CYP2A6*9 in the Ghanaian subjects. No subject homozygous for the CYP2A6*4A allele, a whole gene deletion type of polymorphism prevalent among Orientals, was found. Furthermore, CYP2A6 variants such as *2, *5, *6, *7, *8, *10 and *11 were absent in these black African populations. CONCLUSIONS: This study provides, for the first time, the results of the analysis of CYP2A6 allele frequency in black African populations and confirms large ethnic differences in the polymorphic CYP2A6 gene.
Authors: N Ariyoshi; Y Takahashi; M Miyamoto; Y Umetsu; S Daigo; T Tateishi; S Kobayashi; Y Mizorogi; M A Loriot; I Stücker; P Beaune; M Kinoshita; T Kamataki Journal: Pharmacogenetics Date: 2000-11
Authors: M Oscarson; R A McLellan; H Gullstén; J A Agúndez; J Benítez; A Rautio; H Raunio; O Pelkonen; M Ingelman-Sundberg Journal: FEBS Lett Date: 1999-10-29 Impact factor: 4.124
Authors: M Pitarque; O von Richter; B Oke; H Berkkan; M Oscarson; M Ingelman-Sundberg Journal: Biochem Biophys Res Commun Date: 2001-06-08 Impact factor: 3.575
Authors: Ellen M McDonagh; Catherine Wassenaar; Sean P David; Rachel F Tyndale; Russ B Altman; Michelle Whirl-Carrillo; Teri E Klein Journal: Pharmacogenet Genomics Date: 2012-09 Impact factor: 2.089
Authors: Keneuoe Cecilia Nthontho; Andrew Khulekani Ndlovu; Kirthana Sharma; Ishmael Kasvosve; Daniel Louis Hertz; Giacomo Maria Paganotti Journal: Pharmgenomics Pers Med Date: 2022-06-21
Authors: Awewura Kwara; Margaret Lartey; Kwamena W Sagoe; Naser L Rzek; Michael H Court Journal: Br J Clin Pharmacol Date: 2009-04 Impact factor: 4.335