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Literature DB >> 15659695

Characterization of phospholipase activity of the Pseudomonas aeruginosa type III cytotoxin, ExoU.

Hiromi Sato¹, Jimmy B Feix, Cecilia J Hillard, Dara W Frank.

Abstract

Recombinant ExoU (rExoU) and yeast extract were used to optimize an in vitro phospholipase assay as a basis for identifying the mechanism for enzyme activation and substrate specificity. Our results support a model in which a eukaryotic protein cofactor or complex facilitates the interaction of rExoU with phospholipid substrates.

Entities: Chemical Species

Mesh：

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Year: 2005 PMID： 15659695 PMCID： PMC545705 DOI： 10.1128/JB.187.3.1192-1195.2005

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

21 in total

1. Lysophospholipase A activity of Pseudomonas aeruginosa type III secretory toxin ExoU.

Authors: Miki Tamura; Temitayo Ajayi; Leonard R Allmond; Kiyoshi Moriyama; Jeanine P Wiener-Kronish; Teiji Sawa
Journal: Biochem Biophys Res Commun Date: 2004-04-02 Impact factor: 3.575

2. Action of phospholipase A2 on unmodified phosphatidylcholine bilayers: organizational defects are preferred sites of action.

Authors: G C Upreti; M K Jain
Journal: J Membr Biol Date: 1980-07-15 Impact factor: 1.843

3. Characterization of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, patatin, by cloning and abundant expression in a baculovirus vector.

Authors: D L Andrews; B Beames; M D Summers; W D Park
Journal: Biochem J Date: 1988-05-15 Impact factor: 3.857

4. Cloning, expression, purification and characterization of patatin, a novel phospholipase A.

Authors: H J Hirschberg; J W Simons; N Dekker; M R Egmond
Journal: Eur J Biochem Date: 2001-10

Review 5. ExoU is a potent intracellular phospholipase.

Authors: Hiromi Sato; Dara W Frank
Journal: Mol Microbiol Date: 2004-09 Impact factor: 3.501

6. A novel arachidonic acid-selective cytosolic PLA2 contains a Ca(2+)-dependent translocation domain with homology to PKC and GAP.

Authors: J D Clark; L L Lin; R W Kriz; C S Ramesha; L A Sultzman; A Y Lin; N Milona; J L Knopf
Journal: Cell Date: 1991-06-14 Impact factor: 41.582

7. cPLA2 is phosphorylated and activated by MAP kinase.

Authors: L L Lin; M Wartmann; A Y Lin; J L Knopf; A Seth; R J Davis
Journal: Cell Date: 1993-01-29 Impact factor: 41.582

8. Pseudomonas aeruginosa exoenzyme S requires a eukaryotic protein for ADP-ribosyltransferase activity.

Authors: J Coburn; A V Kane; L Feig; D M Gill
Journal: J Biol Chem Date: 1991-04-05 Impact factor: 5.157

9. Pseudomonas aeruginosa ExoU, a toxin transported by the type III secretion system, kills Saccharomyces cerevisiae.

Authors: Shira D P Rabin; Alan R Hauser
Journal: Infect Immun Date: 2003-07 Impact factor: 3.441

10. Enzymatic hydrolysis of short-chain lecithin/long-chain phospholipid unilamellar vesicles: sensitivity of phospholipases to matrix phase state.

Authors: N E Gabriel; N V Agman; M F Roberts
Journal: Biochemistry Date: 1987-11-17 Impact factor: 3.162

32 in total

1. Traversal of multilayered corneal epithelia by cytotoxic Pseudomonas aeruginosa requires the phospholipase domain of exoU.

Authors: Julio C Ramirez; Suzanne M J Fleiszig; Aaron B Sullivan; Connie Tam; Roya Borazjani; David J Evans
Journal: Invest Ophthalmol Vis Sci Date: 2012-01-25 Impact factor: 4.799

Characterization of phospholipase activity of the Pseudomonas aeruginosa type III cytotoxin, ExoU.

1. Lysophospholipase A activity of Pseudomonas aeruginosa type III secretory toxin ExoU.

2. Action of phospholipase A2 on unmodified phosphatidylcholine bilayers: organizational defects are preferred sites of action.

3. Characterization of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, patatin, by cloning and abundant expression in a baculovirus vector.

4. Cloning, expression, purification and characterization of patatin, a novel phospholipase A.

Review 5. ExoU is a potent intracellular phospholipase.

6. A novel arachidonic acid-selective cytosolic PLA2 contains a Ca(2+)-dependent translocation domain with homology to PKC and GAP.

7. cPLA2 is phosphorylated and activated by MAP kinase.

8. Pseudomonas aeruginosa exoenzyme S requires a eukaryotic protein for ADP-ribosyltransferase activity.

9. Pseudomonas aeruginosa ExoU, a toxin transported by the type III secretion system, kills Saccharomyces cerevisiae.

10. Enzymatic hydrolysis of short-chain lecithin/long-chain phospholipid unilamellar vesicles: sensitivity of phospholipases to matrix phase state.

1. Traversal of multilayered corneal epithelia by cytotoxic Pseudomonas aeruginosa requires the phospholipase domain of exoU.

2. Ubiquitin and ubiquitin-modified proteins activate the Pseudomonas aeruginosa T3SS cytotoxin, ExoU.

3. Role of the membrane localization domain of the Pseudomonas aeruginosa effector protein ExoU in cytotoxicity.

4. MexT regulates the type III secretion system through MexS and PtrC in Pseudomonas aeruginosa.

5. A C-terminal domain targets the Pseudomonas aeruginosa cytotoxin ExoU to the plasma membrane of host cells.

6. Pseudolipasin A is a specific inhibitor for phospholipase A2 activity of Pseudomonas aeruginosa cytotoxin ExoU.

7. Induced conformational changes in the activation of the Pseudomonas aeruginosa type III toxin, ExoU.

8. In vitro assays to monitor the activity of Pseudomonas aeruginosa Type III secreted proteins.

9. PtrB of Pseudomonas aeruginosa suppresses the type III secretion system under the stress of DNA damage.

10. A sensitive fluorescence-based assay for the detection of ExoU-mediated PLA(2) activity.