BACKGROUND: Atopic dermatitis (AD)-specific genes have not yet been clarified. Objectives To identify gene expression specific to active atopic skin lesions. METHODS: We analysed 23,000 genes in skin biopsy samples from 17 patients with AD and four normal controls using Affymetrix oligonucleotide arrays. RESULTS: Four of the 10 genes with the greatest differences in expression between patients and controls, S100A8 and S100A7 (upregulated), and loricrin and filaggrin (downregulated), were epidermal differentiation genes located on 1q21, a locus previously reported to have a genetic linkage with AD. CONCLUSIONS: Our results, showing downregulation of the cornified envelope genes and upregulation of the alternative keratinization pathway, are the first to suggest abnormal epidermal differentiation and defective defences as key abnormalities in AD.
BACKGROUND:Atopic dermatitis (AD)-specific genes have not yet been clarified. Objectives To identify gene expression specific to active atopic skin lesions. METHODS: We analysed 23,000 genes in skin biopsy samples from 17 patients with AD and four normal controls using Affymetrix oligonucleotide arrays. RESULTS: Four of the 10 genes with the greatest differences in expression between patients and controls, S100A8 and S100A7 (upregulated), and loricrin and filaggrin (downregulated), were epidermal differentiation genes located on 1q21, a locus previously reported to have a genetic linkage with AD. CONCLUSIONS: Our results, showing downregulation of the cornified envelope genes and upregulation of the alternative keratinization pathway, are the first to suggest abnormal epidermal differentiation and defective defences as key abnormalities in AD.
Authors: Tolly G Epstein; Grace K LeMasters; David I Bernstein; Mark B Ericksen; Lisa J Martin; Patrick H Ryan; Jocelyn M Biagini Myers; Melinda S Butsch Kovacic; Mark A Lindsey; Hua He; Tiina Reponen; Manuel S Villareal; James E Lockey; Cheryl K Bernstein; Gurjit K Khurana Hershey Journal: Ann Allergy Asthma Immunol Date: 2012-03 Impact factor: 6.347