OBJECTIVE: To establish a HPLC method for determining orientin and vitexin in Trollius chinesis preparation. METHOD: The separation was carried out on Hypersil ODS column with a mixture of acetonitrile-1% acetic acid solution (15:85) as the mobile phase, at a flow rate of 1.0 mL x min(-1). The detection wavelength was set 340 nm. RESULT: Orientin showed a good linear relationship in the range of 8.42-84.2 microg x mL(-1) (r = 0.9999) and the average recovery is 98.3%, RSD is 0.8%. Vitexin showed a good linear relationship in the range of 8.07-80.7 microg x mL(-1) (r = 0.9999) and the average recovery is 102.4%. RSD is 1.7%. CONCLUSION: The method is sensitive, specific, and accurate with a good repeatability and can be used for the quality control of Trollius chinesis preparation.
OBJECTIVE: To establish a HPLC method for determining orientin and vitexin in Trollius chinesis preparation. METHOD: The separation was carried out on Hypersil ODS column with a mixture of acetonitrile-1% acetic acid solution (15:85) as the mobile phase, at a flow rate of 1.0 mL x min(-1). The detection wavelength was set 340 nm. RESULT: Orientin showed a good linear relationship in the range of 8.42-84.2 microg x mL(-1) (r = 0.9999) and the average recovery is 98.3%, RSD is 0.8%. Vitexin showed a good linear relationship in the range of 8.07-80.7 microg x mL(-1) (r = 0.9999) and the average recovery is 102.4%. RSD is 1.7%. CONCLUSION: The method is sensitive, specific, and accurate with a good repeatability and can be used for the quality control of Trollius chinesis preparation.
Authors: Ahmed Ibrahim Foudah; Prawez Alam; Y T Kamal; Saleh Ibrahim Alqasoumi; Mohammed Hamed Alqarni; Samir A Ross; Hasan Soliman Yusufoglu Journal: Saudi Pharm J Date: 2019-11-08 Impact factor: 4.330