The role of ascorbic acid in mesenchymal differentiation.

Survival of all higher vertebrates requires that they either synthesize vitamin C (ascorbic acid) or obtain it from their diet. The role of ascorbic acid as a reductant for the iron prosthetic group of hydroxylase enzymes involved in collagen biosynthesis is well established. In contrast, the relationship between the biochemical functions of ascorbic acid and the broad defects in connective tissue formation associated with vitamin C deficiency is less obvious. This review will develop the hypothesis that vitamin C is required for the differentiation of mesenchyme-derived connective tissues such as muscle, cartilage, and bone. It is proposed that the collagen matrix produced by ascorbic acid-treated cells provides a permissive environment for tissue-specific gene expression.