BACKGROUND: We previously reported that relative expression of E-cadherin, matrix metalloproteinases (MMPs)-2 and -9, and vascular endothelial growth factor (VEGF)/vascular permeability factor in radical prostatectomy specimens (RP) can distinguish organ-confined cancers from advanced prostate cancers. Here, we evaluate the expression of interleukin-8 (IL-8) and basic fibroblast growth factor (bFGF), two other genes involved in angiogenesis and metastasis, in RP specimens. METHODS: The expression level of IL-8 and bFGF mRNA in the invasive edge of 41 prostate cancers of different stages was determined using a rapid colorimetric in situ hybridization (ISH) technique. Gene expression levels of IL-8 and bFGF were correlated with the Gleason score and pathologic stage to ascertain their relationship to prostate cancer progression. RESULTS: The expression of IL-8 and bFGF genes was detected by ISH in histologically normal prostate gland epithelium as well as in glands with foci of cancer. Increased mRNA expression of IL-8 was associated with both the Gleason score and pathologic stage of tumors and distinguished organ-confined from non-confined tumors (P = 0.002). In contrast, the expression of bFGF mRNA did not correlate with the Gleason score or pathologic stage. CONCLUSIONS: Overexpression of Il-8 mRNA, but not bFGF mRNA, in RP specimens is directly associated with progression of prostate cancer.
BACKGROUND: We previously reported that relative expression of E-cadherin, matrix metalloproteinases (MMPs)-2 and -9, and vascular endothelial growth factor (VEGF)/vascular permeability factor in radical prostatectomy specimens (RP) can distinguish organ-confined cancers from advanced prostate cancers. Here, we evaluate the expression of interleukin-8 (IL-8) and basic fibroblast growth factor (bFGF), two other genes involved in angiogenesis and metastasis, in RP specimens. METHODS: The expression level of IL-8 and bFGF mRNA in the invasive edge of 41 prostate cancers of different stages was determined using a rapid colorimetric in situ hybridization (ISH) technique. Gene expression levels of IL-8 and bFGF were correlated with the Gleason score and pathologic stage to ascertain their relationship to prostate cancer progression. RESULTS: The expression of IL-8 and bFGF genes was detected by ISH in histologically normal prostate gland epithelium as well as in glands with foci of cancer. Increased mRNA expression of IL-8 was associated with both the Gleason score and pathologic stage of tumors and distinguished organ-confined from non-confined tumors (P = 0.002). In contrast, the expression of bFGF mRNA did not correlate with the Gleason score or pathologic stage. CONCLUSIONS: Overexpression of Il-8 mRNA, but not bFGF mRNA, in RP specimens is directly associated with progression of prostate cancer.
Authors: Daniel J Caruso; Adrienne J K Carmack; Vinata B Lokeshwar; Robert C Duncan; Mark S Soloway; Bal L Lokeshwar Journal: Clin Cancer Res Date: 2008-07-01 Impact factor: 12.531
Authors: Shizhi He; Shuangba He; Chun-Hao Chen; Sylvie Deborde; Richard L Bakst; Natalya Chernichenko; William F McNamara; Sei Young Lee; Fernando Barajas; Zhenkun Yu; Hikmat A Al-Ahmadie; Richard J Wong Journal: Mol Cancer Res Date: 2014-10-13 Impact factor: 5.852
Authors: Ke Chen; Kongming Wu; Xuanmao Jiao; Liping Wang; Xiaoming Ju; Min Wang; Gabriele Di Sante; Shaohua Xu; Qiong Wang; Kevin Li; Xin Sun; Congwen Xu; Zhiping Li; Mathew C Casimiro; Adam Ertel; Sankar Addya; Peter A McCue; Michael P Lisanti; Chenguang Wang; Richard J Davis; Graeme Mardon; Richard G Pestell Journal: Cancer Res Date: 2015-03-13 Impact factor: 12.701