Literature DB >> 15644178

Delivery of small interfering RNA to mammalian cells in culture by using cationic lipid/polymer-based transfection reagents.

Robert M Brazas1, James E Hagstrom.   

Abstract

RNA interference (RNAi) has become a powerful tool for the knockdown of target gene expression and subsequent phenotypic analysis of gene function in mammalian cells in culture. Critical to the success of any small inhibitory RNA (siRNA)-mediated RNAi knockdown in mammalian cells is the efficient delivery of the siRNA to those cells. This chapter describes the use of popular cationic lipid?polymer-based transfection reagents for in vitro siRNA delivery and includes a general protocol with special emphasis on key transfection parameters important to the success of siRNA delivery.

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Year:  2005        PMID： 15644178     DOI： 10.1016/S0076-6879(04)92007-1

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  9 in total

1.  Msx2 exerts bone anabolism via canonical Wnt signaling.

Authors:  Su-Li Cheng; Jian-Su Shao; Jun Cai; Oscar L Sierra; Dwight A Towler
Journal:  J Biol Chem       Date:  2008-05-15       Impact factor: 5.157

2.  Processing of abasic DNA clusters in hApeI-silenced primary fibroblasts exposed to low doses of X-irradiation.

Authors:  Prolay Das; Betsy M Sutherland
Journal:  J Biosci       Date:  2011-03       Impact factor: 1.826

3.  Self-assembled lamellar complexes of siRNA with lipidic aminoglycoside derivatives promote efficient siRNA delivery and interference.

Authors:  Léa Desigaux; Matthieu Sainlos; Olivier Lambert; Raphael Chevre; Emilie Letrou-Bonneval; Jean-Pierre Vigneron; Pierre Lehn; Jean-Marie Lehn; Bruno Pitard
Journal:  Proc Natl Acad Sci U S A       Date:  2007-10-08       Impact factor: 11.205

4.  Targeting V600EB-Raf and Akt3 using nanoliposomal-small interfering RNA inhibits cutaneous melanocytic lesion development.

Authors:  Melissa A Tran; Raghavendra Gowda; Arati Sharma; Eun-Joo Park; James Adair; Mark Kester; Nadine Barrie Smith; Gavin P Robertson
Journal:  Cancer Res       Date:  2008-09-15       Impact factor: 12.701

5.  New-Generation Benzimidazole-Based Plasmid Delivery Reagents with High Transfection Efficiencies on the Mammalian Cells.

Authors:  Furkan Ayaz; Ronak Haj Ersan; Burak Kuzu; Oztekin Algul
Journal:  In Vitro Cell Dev Biol Anim       Date:  2020-01-02       Impact factor: 2.416

6.  Plasticity of subventricular zone neuroprogenitors in MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) mouse model of Parkinson's disease involves cross talk between inflammatory and Wnt/β-catenin signaling pathways: functional consequences for neuroprotection and repair.

Authors:  Francesca L'Episcopo; Cataldo Tirolo; Nunzio Testa; Salvatore Caniglia; Maria C Morale; Michela Deleidi; Maria F Serapide; Stefano Pluchino; Bianca Marchetti
Journal:  J Neurosci       Date:  2012-02-08       Impact factor: 6.167

7.  A Wnt1 regulated Frizzled-1/β-Catenin signaling pathway as a candidate regulatory circuit controlling mesencephalic dopaminergic neuron-astrocyte crosstalk: Therapeutical relevance for neuron survival and neuroprotection.

Authors:  Francesca L'episcopo; Maria F Serapide; Cataldo Tirolo; Nunzio Testa; Salvatore Caniglia; Maria C Morale; Stefano Pluchino; Bianca Marchetti
Journal:  Mol Neurodegener       Date:  2011-07-13       Impact factor: 14.195

Review 8.  RNA interference: from gene silencing to gene-specific therapeutics.

Authors:  Ray K M Leung; Paul A Whittaker
Journal:  Pharmacol Ther       Date:  2005-08       Impact factor: 12.310

9.  siRNA-containing liposomes modified with polyarginine effectively silence the targeted gene.

Authors:  Chunling Zhang; Ning Tang; XingJun Liu; Wei Liang; Wei Xu; Vladimir P Torchilin
Journal:  J Control Release       Date:  2006-03-20       Impact factor: 9.776
  9 in total

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