L N Ten1, W-T Im, M-K Kim, S-T Lee. 1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Kuseong-Dong, Yuseong-Gu, Daejeon, Korea.
Abstract
AIMS: To develop a plate assay for simultaneous screening of polysaccharide-degrading and protein-degrading micro-organisms. METHODS AND RESULTS: A plate assay, based on the visible solubilization of small substrate particles and the formation of haloes on Petri dishes, containing a mixture of diversely coloured insoluble polysaccharides and dye-labelled collagen as chromogenic substrates, was developed. This method was successfully applied for isolating the diverse polysaccharide- and/or protein-degrading bacteria from soil and sludge samples. Selected strains were identified using 16S rDNA partial sequencing; most of them belong to the genera Bacillus, Cellulomonas and Cellulosimicrobium. CONCLUSIONS: This novel approach provides unique and valuable information for direct primary screening when the target of selection is micro-organisms exhibiting protein-degrading activity, polysaccharide-degrading activity or a specific combination of them. SIGNIFICANCE AND IMPACT OF THE STUDY: This plate assay is convenient and easy to perform, rapid, and more adaptable for screening of a large number of samples, compared with other existing methods in the literature.
AIMS: To develop a plate assay for simultaneous screening of polysaccharide-degrading and protein-degrading micro-organisms. METHODS AND RESULTS: A plate assay, based on the visible solubilization of small substrate particles and the formation of haloes on Petri dishes, containing a mixture of diversely coloured insoluble polysaccharides and dye-labelled collagen as chromogenic substrates, was developed. This method was successfully applied for isolating the diverse polysaccharide- and/or protein-degrading bacteria from soil and sludge samples. Selected strains were identified using 16S rDNA partial sequencing; most of them belong to the genera Bacillus, Cellulomonas and Cellulosimicrobium. CONCLUSIONS: This novel approach provides unique and valuable information for direct primary screening when the target of selection is micro-organisms exhibiting protein-degrading activity, polysaccharide-degrading activity or a specific combination of them. SIGNIFICANCE AND IMPACT OF THE STUDY: This plate assay is convenient and easy to perform, rapid, and more adaptable for screening of a large number of samples, compared with other existing methods in the literature.
Authors: Benedikt Leis; Simon Heinze; Angel Angelov; Vu Thuy Trang Pham; Andrea Thürmer; Mohamed Jebbar; Peter N Golyshin; Wolfgang R Streit; Rolf Daniel; Wolfgang Liebl Journal: Front Bioeng Biotechnol Date: 2015-07-01
Authors: Stjepan Krešimir Kračun; Julia Schückel; Bjørge Westereng; Lisbeth Garbrecht Thygesen; Rune Nygaard Monrad; Vincent G H Eijsink; William George Tycho Willats Journal: Biotechnol Biofuels Date: 2015-04-23 Impact factor: 6.040