PURPOSE: This investigation aimed at determining whether a collagenolytic matrix metalloproteinase (MMP-8) present in the oral fluids might attack demineralized dentin and thereby affect the potential for remineralization. MATERIALS AND METHODS: Dentin specimens from freshly extracted third molars were demineralized for 14 days in vitro and then remineralized for 7 days in the presence or absence of activated MMP-8. Following treatment the mineral level of the specimens was assessed from the outer dentinal surface using Electron Probe Micro Analysis (EPMA). RESULTS: The findings suggested that the addition of activated MMP-8 to the remineralizing solution led to reduced remineralization in the surface of the dentin. There appeared to be no effect on the remineralization from any dentin bound MMP activity. CONCLUSION: It appears that MMP-8 may negatively influence the remineralization of demineralized dentin.
PURPOSE: This investigation aimed at determining whether a collagenolytic matrix metalloproteinase (MMP-8) present in the oral fluids might attack demineralized dentin and thereby affect the potential for remineralization. MATERIALS AND METHODS: Dentin specimens from freshly extracted third molars were demineralized for 14 days in vitro and then remineralized for 7 days in the presence or absence of activated MMP-8. Following treatment the mineral level of the specimens was assessed from the outer dentinal surface using Electron Probe Micro Analysis (EPMA). RESULTS: The findings suggested that the addition of activated MMP-8 to the remineralizing solution led to reduced remineralization in the surface of the dentin. There appeared to be no effect on the remineralization from any dentin bound MMP activity. CONCLUSION: It appears that MMP-8 may negatively influence the remineralization of demineralized dentin.
Authors: Leo Tjäderhane; Fabio D Nascimento; Lorenzo Breschi; Annalisa Mazzoni; Ivarne L S Tersariol; Saulo Geraldeli; Arzu Tezvergil-Mutluay; Marcela R Carrilho; Ricardo M Carvalho; Franklin R Tay; David H Pashley Journal: Dent Mater Date: 2012-08-16 Impact factor: 5.304