| Literature DB >> 1564324 |
A Ogata1, H Tagoh, T Lee, T Kuritani, Y Takahara, T Shimamura, H Ikegami, M Kurimoto, K Yoshizaki, T Kishimoto.
Abstract
Non-isotopic immunoassays for human tumor necrosis factor alpha (TNF alpha) and human interleukin-6 (IL-6) were established by employing the dissociation-enhanced lanthanide fluoroimmunoassay (DELFIA) system based on the time-resolved fluoroimmunoassay technique with europium-labeled antibody. Compared to enzyme-linked immunosorbent assays and bioassays, the sensitivity and range of measurement were significantly increased by applying the DELFIA systems to TNF alpha and IL-6. TNF alpha was measurable from 100 fg/ml to 10 ng/ml with the TNF alpha-DELFIA and IL-6 was measurable from 100 fg/ml to 1 ng/ml with the IL-6-DELFIA.Entities:
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Year: 1992 PMID： 1564324 DOI： 10.1016/0022-1759(92)90153-k
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303