Literature DB >> 1564321

Biosynthetic radiolabeling of bacterial lipopolysaccharide to high specific activity.

R S Munford1, L C DeVeaux, J E Cronan, P D Rick.   

Abstract

We describe a method for producing radiolabeled lipopolysaccharide (LPS) by incorporating [3H]acetate into an aceEF, gltA strain of Escherichia coli K12. The LPS has substantially greater specific radioactivity (2 microCi per microgram LPS, or approximately 8 Ci/mmol) than has been reported previously for biosynthetically radiolabeled LPS. The 3H is incorporated into the fatty acyl chains of the lipid A moiety. LPS prepared by this method has several attractive features for biological studies, including native structure and bioactivity, long radioactive half-life, and high specific activity.

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Year:  1992        PMID： 1564321     DOI： 10.1016/0022-1759(92)90164-o

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  13 in total

1.  ABCA1 promotes the efflux of bacterial LPS from macrophages and accelerates recovery from LPS-induced tolerance.

Authors:  Patricia A Thompson; Karine C Gauthier; Alan W Varley; Richard L Kitchens
Journal:  J Lipid Res       Date:  2010-05-15       Impact factor: 5.922

2.  Toll-like receptor agonists promote prolonged triglyceride storage in macrophages.

Authors:  Ying-ling Huang; Joel Morales-Rosado; Jessica Ray; Timothy G Myers; Terry Kho; Mingfang Lu; Robert S Munford
Journal:  J Biol Chem       Date:  2013-12-11       Impact factor: 5.157

3.  Plasma CD14 decreases monocyte responses to LPS by transferring cell-bound LPS to plasma lipoproteins.

Authors:  R L Kitchens; P A Thompson; S Viriyakosol; G E O'Keefe; R S Munford
Journal:  J Clin Invest       Date:  2001-08       Impact factor: 14.808

4.  Deacylation of purified lipopolysaccharides by cellular and extracellular components of a sterile rabbit peritoneal inflammatory exudate.

Authors:  Y Weinrauch; S S Katz; R S Munford; P Elsbach; J Weiss
Journal:  Infect Immun       Date:  1999-07       Impact factor: 3.441

5.  Lipopolysaccharide activation of human endothelial and epithelial cells is mediated by lipopolysaccharide-binding protein and soluble CD14.

Authors:  J Pugin; C C Schürer-Maly; D Leturcq; A Moriarty; R J Ulevitch; P S Tobias
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205

6.  Lipopolysaccharide (LPS) partial structures inhibit responses to LPS in a human macrophage cell line without inhibiting LPS uptake by a CD14-mediated pathway.

Authors:  R L Kitchens; R J Ulevitch; R S Munford
Journal:  J Exp Med       Date:  1992-08-01       Impact factor: 14.307

7.  Apolipoprotein A-II augments monocyte responses to LPS by suppressing the inhibitory activity of LPS-binding protein.

Authors:  Patricia A Thompson; Jimmy F P Berbée; Patrick C N Rensen; Richard L Kitchens
Journal:  Innate Immun       Date:  2008-12       Impact factor: 2.680

8.  Isolation of an endotoxin-MD-2 complex that produces Toll-like receptor 4-dependent cell activation at picomolar concentrations.

Authors:  Theresa L Gioannini; Athmane Teghanemt; DeSheng Zhang; Nathan P Coussens; Wendie Dockstader; S Ramaswamy; Jerrold P Weiss
Journal:  Proc Natl Acad Sci U S A       Date:  2004-03-09       Impact factor: 11.205

9.  Identification of acyloxyacyl hydrolase, a lipopolysaccharide-detoxifying enzyme, in the murine urinary tract.

Authors:  J Amelia Feulner; Mingfang Lu; John M Shelton; Mei Zhang; James A Richardson; Robert S Munford
Journal:  Infect Immun       Date:  2004-06       Impact factor: 3.441

10.  Host inactivation of bacterial lipopolysaccharide prevents prolonged tolerance following gram-negative bacterial infection.

Authors:  Mingfang Lu; Alan W Varley; Shoichiro Ohta; John Hardwick; Robert S Munford
Journal:  Cell Host Microbe       Date:  2008-09-11       Impact factor: 21.023
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