Literature DB >> 15643012

Comparison of a new, affordable flow cytometric method and the manual magnetic bead technique for CD4 T-lymphocyte counting in a northern Nigerian setting.

Godwin E Imade1, Bitrus Badung, Sunday Pam, Oche Agbaji, Daniel Egah, Atiene S Sagay, Jean-Louis Sankalé, Saidi Kapiga, John Idoko, Phyllis Kanki.   

Abstract

We compared two techniques for CD4 T-lymphocyte counting: flow cytometry (Cyflow) and magnetic beads (Dynabead). Similar results with good correlation were obtained from the 40 adult blood samples counted (P=0.057, r=0.93). The Cyflow technique is more precise and cost-effective than the Dynabead method ($3 to $5 versus $12 to $22 per test, respectively), since as many as 200 samples can be measured per day.

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Mesh：

Year:  2005        PMID： 15643012      PMCID： PMC540213          DOI： 10.1128/CDLI.12.1.224-227.2005

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  10 in total

1.  Multisite comparison of CD4 and CD8 T-lymphocyte counting by single- versus multiple-platform methodologies: evaluation of Beckman Coulter flow-count fluorospheres and the tetraONE system. The NIAID DAIDS New Technologies Evaluation Group.

Authors:  K A Reimann; M R O'Gorman; J Spritzler; C L Wilkening; D E Sabath; K Helm; D E Campbell
Journal:  Clin Diagn Lab Immunol       Date:  2000-05

2.  Evaluation of TruCount absolute-count tubes for determining CD4 and CD8 cell numbers in human immunodeficiency virus-positive adults. Site Investigators and The NIAID DAIDS New Technologies Evaluation Group.

Authors:  C T Schnizlein-Bick; J Spritzler; C L Wilkening; J K Nicholson; M R O'Gorman
Journal:  Clin Diagn Lab Immunol       Date:  2000-05

3.  Prices of CD4 assays and viral load tests must be reduced for developing countries.

Authors:  C Bartholomew
Journal:  BMJ       Date:  2001-10-06

4.  Affordable CD4(+) T-cell counts on 'single-platform' flow cytometers I. Primary CD4 gating.

Authors:  G Janossy; I Jani; W Göhde
Journal:  Br J Haematol       Date:  2000-12       Impact factor: 6.998

5.  Affordable CD4(+)-T-cell counting by flow cytometry: CD45 gating for volumetric analysis.

Authors:  George Janossy; Ilesh V Jani; Nicholas J Bradley; Arsene Bikoue; Tim Pitfield; Debbie K Glencross
Journal:  Clin Diagn Lab Immunol       Date:  2002-09

6.  Evaluation of the FACScount, TRAx CD4 and Dynabeads methods for CD4 lymphocyte determination.

Authors:  E F Lyamuya; C Kagoma; E C Mbena; W K Urassa; K Pallangyo; F S Mhalu; G Biberfeld
Journal:  J Immunol Methods       Date:  1996-09-09       Impact factor: 2.303

7.  High-grade loss of leukocytes and hematopoietic progenitor cells caused by erythrocyte-lysing procedures for flow cytometric analyses.

Authors:  Burkhard Greve; Constanze Beller; Uwe Cassens; Walter Sibrowski; Erhard Severin; Wolfgang Göhde
Journal:  J Hematother Stem Cell Res       Date:  2003-06

8.  A manual bead assay for the determination of absolute CD4+ and CD8+ lymphocyte counts in human immunodeficiency virus-infected individuals.

Authors:  A V Carella; M W Moss; V Provost; T C Quinn
Journal:  Clin Diagn Lab Immunol       Date:  1995-09

9.  Monitoring of human immunodeficiency virus infection in resource-constrained countries.

Authors:  Suzanne Crowe; Shannon Turnbull; Robert Oelrichs; Amanda Dunne
Journal:  Clin Infect Dis       Date:  2003-07-01       Impact factor: 9.079

10.  Successful implementation of a low-cost method for enumerating CD4+ T lymphocytes in resource-limited settings: the ANRS 12-26 study.

Authors:  Serge Diagbouga; Corine Chazallon; Michel D Kazatchkine; Philippe Van de Perre; André Inwoley; Souleymane M'Boup; Mireille Prince David; Aoua Thiéro Ténin; Robert Soudré; Jean-Pierre Aboulker; Laurence Weiss
Journal:  AIDS       Date:  2003-10-17       Impact factor: 4.177
  10 in total
  8 in total

1.  Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe.

Authors:  Justen Manasa; Hazvineyi Musabaike; Collen Masimirembwa; Eileen Burke; Ruedi Luthy; James Mudzori
Journal:  Clin Vaccine Immunol       Date:  2007-01-31

2.  Fast sorting of CD4+ T cells from whole blood using glass microbubbles.

Authors:  Chia-Hsien Hsu; Chihchen Chen; Daniel Irimia; Mehmet Toner
Journal:  Technology (Singap World Sci)       Date:  2015-03

3.  Discerning in vitro pharmacodynamics from OD measurements: A model-based approach.

Authors:  Iordanis Kesisoglou; Vincent H Tam; Andrew P Tomaras; Michael Nikolaou
Journal:  Comput Chem Eng       Date:  2021-12-16       Impact factor: 3.845

4.  Affordable flow cytometry for enumeration of absolute CD4+ T-lymphocytes to identify subtype C HIV-1 infected adults requiring antiretroviral therapy (ART) and monitoring response to ART in a resource-limited setting.

Authors:  Lynn S Zijenah; Gerard Kadzirange; Simon Madzime; Margaret Borok; Chiedza Mudiwa; Ocean Tobaiwa; Mary Mucheche; Simbarashe Rusakaniko; David A Katzenstein
Journal:  J Transl Med       Date:  2006-08-14       Impact factor: 5.531

Review 5.  CD4 enumeration technologies: a systematic review of test performance for determining eligibility for antiretroviral therapy.

Authors:  Rosanna W Peeling; Kimberly A Sollis; Sarah Glover; Suzanne M Crowe; Alan L Landay; Ben Cheng; David Barnett; Thomas N Denny; Thomas J Spira; Wendy S Stevens; Siobhan Crowley; Shaffiq Essajee; Marco Vitoria; Nathan Ford
Journal:  PLoS One       Date:  2015-03-19       Impact factor: 3.240

6.  Evaluation of CD4+ T cells in HIV patients presenting with malaria at the University of Ilorin Teaching Hospital Nigeria.

Authors:  Olajide O Agbede; Temitope O Ajiboye; Olatunji M Kolawole; Samuel A Babatunde; Louis O Odeigha
Journal:  EXCLI J       Date:  2010-04-26       Impact factor: 4.068

7.  Near real-time enumeration of live and dead bacteria using a fibre-based spectroscopic device.

Authors:  Fang Ou; Cushla McGoverin; Simon Swift; Frédérique Vanholsbeeck
Journal:  Sci Rep       Date:  2019-03-18       Impact factor: 4.379

8.  Predictions of CD4 lymphocytes' count in HIV patients from complete blood count.

Authors:  Javier O Rodríguez; Signed E Prieto; Catalina Correa; Carlos E Pérez; Jessica T Mora; Juan Bravo; Yolanda Soracipa; Luisa F Alvarez
Journal:  BMC Med Phys       Date:  2013-09-14
  8 in total

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