Literature DB >> 15640056

[An efficient macro-method of genomic DNA isolation from Actinidia chinensis leaves].

Kun-Song Chen1, Fang Li, Chang-Jie Xu, Shang-Long Zhang, Cheng-Xin Fu.   

Abstract

It is a difficult problem to isolate high quality DNA from plants containing a high contents of polyphenolics and polysaccharose, such as Actinidia plant. The protocol described in this paper is a modified CTAB (hexadecyltrimethylammonium bromide) method. High quality genomic DNA can be isolated from Actinidia plant using the improved method. The DNA is good enough for Southern blot and other uses in DNA research. The protocol is also efficient for quick and macro-DNA extraction.

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Year:  2004        PMID: 15640056

Source DB:  PubMed          Journal:  Yi Chuan        ISSN: 0253-9772


  4 in total

1.  An Anthocyanin-Related Glutathione S-Transferase, MrGST1, Plays an Essential Role in Fruit Coloration in Chinese Bayberry (Morella rubra).

Authors:  Lei Xue; Xiaorong Huang; Zehuang Zhang; Qihua Lin; Qiuzhen Zhong; Yun Zhao; Zhongshan Gao; Changjie Xu
Journal:  Front Plant Sci       Date:  2022-06-08       Impact factor: 6.627

2.  PpGST1, an anthocyanin-related glutathione S-transferase gene, is essential for fruit coloration in peach.

Authors:  Yun Zhao; Weiqi Dong; Yongchao Zhu; Andrew C Allan; Kui Lin-Wang; Changjie Xu
Journal:  Plant Biotechnol J       Date:  2019-11-19       Impact factor: 9.803

3.  Molecular cytogenetic characterization of a new wheat-Thinopyrum intermedium homoeologous group-6 chromosome disomic substitution line with resistance to leaf rust and stripe rust.

Authors:  Xiaojun Zhang; Jianbo Li; Yudi Ge; Haixia Guan; Guangrong Li; Shuwei Zhang; Xiaolu Wang; Xin Li; Zhijian Chang; Peng Zhang; Juqing Jia; Cheng Liu
Journal:  Front Plant Sci       Date:  2022-09-06       Impact factor: 6.627

4.  Standard addition quantitative real-time PCR (SAQPCR): a novel approach for determination of transgene copy number avoiding PCR efficiency estimation.

Authors:  Yuji Huang; Xueren Yin; Changqing Zhu; Weiwei Wang; Donald Grierson; Changjie Xu; Kunsong Chen
Journal:  PLoS One       Date:  2013-01-07       Impact factor: 3.240

  4 in total

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