Genetic variation among epizootic hemorrhagic disease viruses in the southeastern United States: 1978-2001.

The epizootic hemorrhagic disease (EHD) viruses, which are endemic in the southeastern United States, are the causative agents of an important clinical disease in wild and captive ruminants. In order to elucidate the molecular epidemiology of EHD serotype 2, the sequences of portions of the S10 and L2 gene segments (which encode the NS3 viral egress protein and the VP2 surface protein, respectively) of viral isolates made from white-tailed deer over 23 years(1978-2001) were determined and compared using phylogenetic analyses. Both loci demonstrated very little genetic variation among isolates, with a 94-100% nucleotide identity among isolates at the S10 locus (98-100% amino acid conservation), and an 89.1-100% identity among isolates at the L2 locus (87.6-100% amino acid conservation). The grouping of isolates within the resulting cladograms appeared nearly random with respect to time of isolation and geographic origin. One exception to this was a distinctive grouping of some isolates from 1996 to 1997, which formed a separate subclade in both the S10 and L2 cladograms.