OBJECTIVE: Smooth muscle cells (SMCs) involved in intimal hyperplasia during transplant vasculopathy are derived both from the graft and the host. Here, the role of an allogenic immune response in the accumulation of host-derived SMCs in the neointima was explored. METHODS: Infrarenal aorta was transplanted from female F344 to male Lewis rats with or without immunosuppression by cyclosporine A (CsA). Accumulation of host-derived SMCs and inflammatory cells in the grafts, SMC proliferation, and apoptosis were analyzed by immunohistochemistry and real-time polymerase chain reaction (PCR) for the SRY gene. Finally, SMCs were seeded in an allogenic or isogenic manner after balloon injury to carotid arteries and SMC survival was estimated. RESULTS: Proliferating graft SMCs and infiltrating leukocytes were observed in the intima early after transplantation. In parallel, inflammatory cells and immunoglobulins infiltrated the media and apoptosis of medial SMCs occurred, leading to destruction of this layer. CsA decreased the number of SRY+ SMCs in the lesions, restricted medial destruction, and improved survival of allogenic SMCs after seeding in injured arteries. CONCLUSIONS: Development of intimal thickenings during transplant vasculopathy involves an allogenic immune response, which promotes accumulation of host-derived SMCs and apoptosis of resident graft SMCs.
OBJECTIVE: Smooth muscle cells (SMCs) involved in intimal hyperplasia during transplant vasculopathy are derived both from the graft and the host. Here, the role of an allogenic immune response in the accumulation of host-derived SMCs in the neointima was explored. METHODS: Infrarenal aorta was transplanted from female F344 to male Lewis rats with or without immunosuppression by cyclosporine A (CsA). Accumulation of host-derived SMCs and inflammatory cells in the grafts, SMC proliferation, and apoptosis were analyzed by immunohistochemistry and real-time polymerase chain reaction (PCR) for the SRY gene. Finally, SMCs were seeded in an allogenic or isogenic manner after balloon injury to carotid arteries and SMC survival was estimated. RESULTS: Proliferating graft SMCs and infiltrating leukocytes were observed in the intima early after transplantation. In parallel, inflammatory cells and immunoglobulins infiltrated the media and apoptosis of medial SMCs occurred, leading to destruction of this layer. CsA decreased the number of SRY+ SMCs in the lesions, restricted medial destruction, and improved survival of allogenic SMCs after seeding in injured arteries. CONCLUSIONS: Development of intimal thickenings during transplant vasculopathy involves an allogenic immune response, which promotes accumulation of host-derived SMCs and apoptosis of resident graft SMCs.
Authors: Zuzana Tobiasova; Lufeng Zhang; Tai Yi; Linfeng Qin; Thomas D Manes; Sanjay Kulkarni; Marc I Lorber; Frederick C Rodriguez; Je-Min Choi; George Tellides; Jordan S Pober; Ivana Kawikova; Alfred L M Bothwell Journal: Circulation Date: 2011-06-20 Impact factor: 29.690
Authors: Cecilia Söderberg-Nauclér; Piotr Religa; Monika K Grudzinska; Krzysztof Bojakowski; Joanna Soin; Frank Stassen Journal: Herpesviridae Date: 2010-12-23
Authors: Sharan Ananthaseshan; Krzysztof Bojakowski; Mariusz Sacharczuk; Piotr Poznanski; Dominik S Skiba; Lisa Prahl Wittberg; Jordan McKenzie; Anna Szkulmowska; Niclas Berg; Piotr Andziak; Hanna Menkens; Maciej Wojtkowski; Dorota Religa; Fredrik Lundell; Tomasz Guzik; Zbigniew Gaciong; Piotr Religa Journal: Sci Rep Date: 2022-08-11 Impact factor: 4.996
Authors: Piotr Religa; Monika K Grudzinska; Krzysztof Bojakowski; Joanna Soin; Jerzy Nozynski; Michal Zakliczynski; Zbigniew Gaciong; Marian Zembala; Cecilia Söderberg-Nauclér Journal: PLoS One Date: 2009-01-14 Impact factor: 3.240
Authors: Xiaoli Ma; Benjamin Hibbert; Dawn White; Richard Seymour; Stewart C Whitman; Edward R O'Brien Journal: PLoS One Date: 2008-03-26 Impact factor: 3.240