| Literature DB >> 15634958 |
L Zsak1, M V Borca, G R Risatti, A Zsak, R A French, Z Lu, G F Kutish, J G Neilan, J D Callahan, W M Nelson, D L Rock.
Abstract
A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.Entities:
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Year: 2005 PMID: 15634958 PMCID: PMC540100 DOI: 10.1128/JCM.43.1.112-119.2005
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948