Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A quantitative high-throughput endothelial cell migration assay.

Literature DB >> 15634798

A quantitative high-throughput endothelial cell migration assay.

Vladimir Mastyugin¹, Elizabeth McWhinnie, Mark Labow, Frank Buxton.

Abstract

By combining the use of BD Biosciences FluoroBlok membrane-based Boyden chambers with the Cellomics HCS ArrayScan, a more sensitive method for measuring cell migration has been developed. This assay is based on counting nuclei of migrated cells on the bottom of the filter rather than conventional approaches, which use measurement of total well fluorescence. This cell migration assay provides approximately 10-fold increased signal/background compared to conventional approaches and can be used to assess the effects of growth factors on endothelial cell migration and to screen chemical compounds for inhibitory effects on growth factor-mediated endothelial cell migration.

Entities: Gene

Mesh：

Substances：

Year: 2004 PMID： 15634798 DOI： 10.1177/1087057104269495

Source DB: PubMed Journal: J Biomol Screen ISSN： 1087-0571

Keyword Cloud
Cited

12 in total

1. Upcyte® microvascular endothelial cells repopulate decellularized scaffold.

Authors: Katharina Scheller; Iris Dally; Nadja Hartmann; Bernhard Münst; Joris Braspenning; Heike Walles
Journal: Tissue Eng Part C Methods Date: 2012-09-13 Impact factor: 3.056

2. A Unique High-Throughput Assay to Identify Novel Small Molecule Inhibitors of Chemotaxis and Migration.

Authors: Xin-Hua Liao; Alan R Kimmel
Journal: Curr Protoc Cell Biol Date: 2017-03-03

3. The syndecan-4/protein kinase Cα pathway mediates prostaglandin E2-induced extracellular regulated kinase (ERK) activation in endothelial cells and angiogenesis in vivo.

Authors: Federico Corti; Federica Finetti; Marina Ziche; Michael Simons
Journal: J Biol Chem Date: 2013-03-22 Impact factor: 5.157

A quantitative high-throughput endothelial cell migration assay.

1. Upcyte® microvascular endothelial cells repopulate decellularized scaffold.

2. A Unique High-Throughput Assay to Identify Novel Small Molecule Inhibitors of Chemotaxis and Migration.

3. The syndecan-4/protein kinase Cα pathway mediates prostaglandin E2-induced extracellular regulated kinase (ERK) activation in endothelial cells and angiogenesis in vivo.

4. Effects of AFP-172 on COX-2-induced angiogenic activities on human umbilical vein endothelial cells.

5. Restoring vasculogenic potential of endothelial cells from diabetic patients through spheroid formation.

6. Identification of novel pro-migratory, cancer-associated genes using quantitative, microscopy-based screening.

7. Influence of levamisole and other angiogenesis inhibitors on angiogenesis and endothelial cell morphology in vitro.

8. Cell migration and invasion assays as tools for drug discovery.

Review 9. Applications of high content screening in life science research.

10. Trypanosoma cruzi Exploits E- and P-Selectins To Migrate Across Endothelial Cells and Extracellular Matrix Proteins.