Literature DB >> 15634796

Assay for ubiquitin ligase activity: high-throughput screen for inhibitors of HDM2.

I V Davydov1, D Woods, Y J Safiran, P Oberoi, H O Fearnhead, S Fang, J P Jensen, A M Weissman, J H Kenten, K H Vousden.   

Abstract

An assay for the autoubiquitination activity of the E3 ligase HDM2 (Mdm2) was developed and adapted to a high-throughput format to identify inhibitors of this activity. The assay can also be used to measure the activity of other E3s and may be useful in finding both inhibitors and activators of a wide range of different ubiquitin ligases.

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Year:  2004        PMID： 15634796     DOI： 10.1177/1087057104267956

Source DB:  PubMed          Journal:  J Biomol Screen        ISSN： 1087-0571


  11 in total

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