| Literature DB >> 15633249 |
Jian-ping Lu1, Tong-bao Liu, Xiao-yun Yu, Fu-cheng Lin.
Abstract
A mature appressorium cDNA library of rice blast fungus, Magnaporthe grisea, was constructed in a lambdaTriplEx2 vector by SMART cDNA library containing 2.37x10(6) independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.Entities:
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Year: 2005 PMID: 15633249 PMCID: PMC1389628 DOI: 10.1631/jzus.2005.B0132
Source DB: PubMed Journal: J Zhejiang Univ Sci B ISSN: 1673-1581 Impact factor: 3.066