| Literature DB >> 15631452 |
Hatsuo Maeda1, Kayoko Yamamoto, Yoko Nomura, Iho Kohno, Leila Hafsi, Noritsugu Ueda, Shoko Yoshida, Masako Fukuda, Yuka Fukuyasu, Yuji Yamauchi, Norio Itoh.
Abstract
Fluorometric detection of O2-* is performed based on desulfonylation of 3 to the corresponding fluoresceins 4 through nucleophilic substitution, and this fluorescing process is quite specific toward O2-* over H2O2, t-BuOOH, NaOCl, 1O2, HO*, NO*, and ONOO-. Furthermore, effects of glutathione, cytochrome P450 reductase/NADPH, and diaphorase/NADH are relatively small on the fluorescing process of probe 3 with X = Y = F, which is useful to detect O2-* released from neutrophils stimulated by phorbol myristate acetate with satisfactory sensitivity.Entities:
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Year: 2005 PMID： 15631452 DOI： 10.1021/ja047018k
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419