| Literature DB >> 15629470 |
Tim J L Van De Parre1, Wim Martinet, Dorien M Schrijvers, Arnold G Herman, Guido R Y De Meyer.
Abstract
Previous studies demonstrated that macrophages are difficult to transfect. In the present study, we investigated whether J774A.1 macrophages can be efficiently transfected using nucleofector technology. Nucleofection of J774A.1 macrophages with mRNA resulted in transfection efficiencies up to 75% without cell death as compared to control pulsed macrophages. In contrast, introduction of DNA into J774A.1 cells caused apoptosis without expression of the gene of interest. Our results show that mRNA nucleofection is a new high-speed transfection method for macrophages.Entities:
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Year: 2005 PMID： 15629470 DOI： 10.1016/j.bbrc.2004.12.027
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575