AIM: To study the ability of fullerenes C(60) to catalyse the reactions of generation of reactive oxygen species (ROS) in water solution after photoexcitation and to affect the vitality of tumor cells in vitro. METHODS: The number and vitality of cultured Ehrlich carcinoma cells or rat thymocytes were determined using tripane blue, ROS levels were registered using the methods of electron paramagnetic resonance (EPR) spectroscopy and spin traps, photoirradiation of water solution of fullerenes C(60) with visible light was carried out using mercury lamp. RESULTS: Irradiation of water solution of fullerenes C(60) (10(-5) M) was accompanied with generation of ROS with the rate of 10 nMol/ml/min. After addition of irradiated C(60) solution to suspension of thymocytes or ascite cells, the decrease of the number of vital cells by 67 and 58%, respectively, has been registered. CONCLUSION: Photoirradiated water solutions of fullerenes C(60) may be potentially useful for photodynamic therapy of tumors as ROS catalytic system.
AIM: To study the ability of fullerenes C(60) to catalyse the reactions of generation of reactive oxygen species (ROS) in water solution after photoexcitation and to affect the vitality of tumor cells in vitro. METHODS: The number and vitality of cultured Ehrlich carcinoma cells or rat thymocytes were determined using tripane blue, ROS levels were registered using the methods of electron paramagnetic resonance (EPR) spectroscopy and spin traps, photoirradiation of water solution of fullerenes C(60) with visible light was carried out using mercury lamp. RESULTS: Irradiation of water solution of fullerenes C(60) (10(-5) M) was accompanied with generation of ROS with the rate of 10 nMol/ml/min. After addition of irradiated C(60) solution to suspension of thymocytes or ascite cells, the decrease of the number of vital cells by 67 and 58%, respectively, has been registered. CONCLUSION: Photoirradiated water solutions of fullerenes C(60) may be potentially useful for photodynamic therapy of tumors as ROS catalytic system.
Authors: Ying-Ying Huang; Sulbha K Sharma; Tianhong Dai; Hoon Chung; Anastasia Yaroslavsky; Maria Garcia-Diaz; Julie Chang; Long Y Chiang; Michael R Hamblin Journal: Nanotechnol Rev Date: 2012-03 Impact factor: 7.848
Authors: George P Tegos; Tatiana N Demidova; Dennisse Arcila-Lopez; Haeryeon Lee; Tim Wharton; Hariprasad Gali; Michael R Hamblin Journal: Chem Biol Date: 2005-10
Authors: Lianghao Ding; Jackie Stilwell; Tingting Zhang; Omeed Elboudwarej; Huijian Jiang; John P Selegue; Patrick A Cooke; Joe W Gray; Fanqing Frank Chen Journal: Nano Lett Date: 2005-12 Impact factor: 11.189
Authors: Tingting Zhang; Jackie L Stilwell; Daniele Gerion; Lianghao Ding; Omeed Elboudwarej; Patrick A Cooke; Joe W Gray; A Paul Alivisatos; Fanqing Frank Chen Journal: Nano Lett Date: 2006-04 Impact factor: 11.189
Authors: Ying-Ying Huang; Sulbha K Sharma; Rui Yin; Tanupriya Agrawal; Long Y Chiang; Michael R Hamblin Journal: J Biomed Nanotechnol Date: 2014-09 Impact factor: 4.099
Authors: Pawel Mroz; George P Tegos; Hariprasad Gali; Tim Wharton; Tadeusz Sarna; Michael R Hamblin Journal: Photochem Photobiol Sci Date: 2007-10-08 Impact factor: 3.982
Authors: Rui Yin; Min Wang; Ying-Ying Huang; Huang-Chiao Huang; Pinar Avci; Long Y Chiang; Michael R Hamblin Journal: Nanomedicine Date: 2013-12-10 Impact factor: 5.307