Literature DB >> 15626509

Characterization of neuropeptide F and its receptor from the African malaria mosquito, Anopheles gambiae.

Stephen F Garczynski1, Joe W Crim, Mark R Brown.   

Abstract

The genome of Anopheles gambiae contains sequences encoding a neuropeptide F (Ang-NPF) and NPF receptor (Ang-NPFR) related to the neuropeptide Y signaling family. cDNAs for each were cloned and sequenced. Ang-NPFR was stably expressed for radioligand binding analysis. Ang-NPF exhibited high affinity (IC50 approximately 3 nM) membrane binding; NPFs from Aedes aegypti (Aea-NPF) and Drosophila melanogaster (Drm-NPF) were less potent, with the rank order: Ang-NPF>Aea-NPF>Drm-NPF>Drm-NPF8-36. RT-PCR analysis revealed Ang-NPF and Ang-NPFR transcripts in all life stages. Ang-NPF and Ang-NPFR may be strategically positioned for signaling in relation to nutritional status in the African malaria mosquito.

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Year:  2005        PMID: 15626509     DOI: 10.1016/j.peptides.2004.07.014

Source DB:  PubMed          Journal:  Peptides        ISSN: 0196-9781            Impact factor:   3.750


  17 in total

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Journal:  Front Endocrinol (Lausanne)       Date:  2012-11-30       Impact factor: 5.555

3.  Characterization and expression of the short neuropeptide F receptor in the African malaria mosquito, Anopheles gambiae.

Authors:  Stephen F Garczynski; Joe W Crim; Mark R Brown
Journal:  Peptides       Date:  2006-11-30       Impact factor: 3.750

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Authors:  Reinhard Predel; Susanne Neupert; Stephen F Garczynski; Joe W Crim; Mark R Brown; William K Russell; Jörg Kahnt; David H Russell; Ronald J Nachman
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Journal:  Front Physiol       Date:  2021-06-18       Impact factor: 4.566

10.  Feeding and the rhodopsin family g-protein coupled receptors in nematodes and arthropods.

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Journal:  Front Endocrinol (Lausanne)       Date:  2012-12-18       Impact factor: 5.555

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