AIMS: The assessment of desmoplasia by traditional semiquantitative methods does not provide reliable prognostic data. The aim of this study was to quantify desmoplasia by computerised image analysis in primary colorectal carcinomas and to investigate its ability to predict overall survival. METHODS: In total, 112 colorectal adenocarcinomas, with a median follow up of 66 months, were studied. The representative tumour sections were stained by the van Gieson method, which stains collagen rich stroma red. For quantitative histochemical measurement, digital images were analysed by a computerised image analysis program to calculate the percentage of red stained tissue area. The percentage of desmoplasia (PD) was related to conventional clinicopathological prognostic factors and overall survival. RESULTS: The mean (SD) PD was 4.85 (3.37). PD was found to be significantly associated with lymph vessel and venous invasion. By Kaplan-Meier analysis, PD was associated with survival-patients with PD > 4 had a shorter survival than those with PD </= 4. In multivariate analysis, tumour stage, distant metastasis, and PD emerged as independent prognostic factors. CONCLUSION: Desmoplasia measured by image analysis seems to be a significant prognostic indicator in patients with colorectal carcinoma and the improved method described in this study would be useful for routine prognostication.
AIMS: The assessment of desmoplasia by traditional semiquantitative methods does not provide reliable prognostic data. The aim of this study was to quantify desmoplasia by computerised image analysis in primary colorectal carcinomas and to investigate its ability to predict overall survival. METHODS: In total, 112 colorectal adenocarcinomas, with a median follow up of 66 months, were studied. The representative tumour sections were stained by the van Gieson method, which stains collagen rich stroma red. For quantitative histochemical measurement, digital images were analysed by a computerised image analysis program to calculate the percentage of red stained tissue area. The percentage of desmoplasia (PD) was related to conventional clinicopathological prognostic factors and overall survival. RESULTS: The mean (SD) PD was 4.85 (3.37). PD was found to be significantly associated with lymph vessel and venous invasion. By Kaplan-Meier analysis, PD was associated with survival-patients with PD > 4 had a shorter survival than those with PD </= 4. In multivariate analysis, tumour stage, distant metastasis, and PD emerged as independent prognostic factors. CONCLUSION: Desmoplasia measured by image analysis seems to be a significant prognostic indicator in patients with colorectal carcinoma and the improved method described in this study would be useful for routine prognostication.
Authors: C C Compton; L P Fielding; L J Burgart; B Conley; H S Cooper; S R Hamilton; M E Hammond; D E Henson; R V Hutter; R B Nagle; M L Nielsen; D J Sargent; C R Taylor; M Welton; C Willett Journal: Arch Pathol Lab Med Date: 2000-07 Impact factor: 5.534
Authors: M T Dimanche-Boitrel; L Vakaet; P Pujuguet; B Chauffert; M S Martin; A Hammann; F Van Roy; M Mareel; F Martin Journal: Int J Cancer Date: 1994-02-15 Impact factor: 7.396
Authors: Ake Oldberg; Sebastian Kalamajski; Alexei V Salnikov; Linda Stuhr; Matthias Mörgelin; Rolf K Reed; Nils-Erik Heldin; Kristofer Rubin Journal: Proc Natl Acad Sci U S A Date: 2007-08-22 Impact factor: 11.205
Authors: N P West; M Dattani; P McShane; G Hutchins; J Grabsch; W Mueller; D Treanor; P Quirke; H Grabsch Journal: Br J Cancer Date: 2010-04-20 Impact factor: 7.640
Authors: Flávia C Rodrigues-Lisoni; Paulo Peitl; Alessandra Vidotto; Giovana M Polachini; José V Maniglia; Juliana Carmona-Raphe; Bianca R Cunha; Tiago Henrique; Caique F Souza; Rodrigo A P Teixeira; Erica E Fukuyama; Pedro Michaluart; Marcos B de Carvalho; Sonia M Oliani; Eloiza H Tajara; P M Cury; M B de Carvalho; E Dias-Neto; D L A Figueiredo; E E Fukuyama; J F Góis-Filho; A M Leopoldino; R C M Mamede; P Michaluart-Junior; R A Moyses; F G Nóbrega; M P Nóbrega; F D Nunes; E F B Ojopi; L N Serafini; P Severino; A M A Silva; W A Silva; N J F Silveira; S C O M Souza; E H Tajara; V Wünsch-Filho; A Amar; C M Bandeira; M A Braconi; L G Brandão; R M Brandão; A L Canto; M Cerione; R Cicco; M J Chagas; H Chedid; A Costa; B R Cunha; O A Curioni; C S Fortes; S A Franzi; A P Z Frizzera; D Gazito; P E M Guimarães; C M Kaneto; R V M López; R Macarenco; M R Magalhães; C Meneses; A M C Mercante; D G Pinheiro; G M Polachini; A Rapoport; C O Rodini; F C Rodrigues-Lisoni; R V Rodrigues; L Rossi; A R D Santos; M Santos; F Settani; F A M Silva; I T Silva; T B Souza; E Stabenow; J T Takamori; P J Valentim; A Vidotto; F C A Xavier; F Yamagushi; M L Cominato; P M S Correa; G S Mendes; R Paiva; O Ramos; C Silva; M J Silva; M V C Tarlá Journal: BMC Med Genomics Date: 2010-05-04 Impact factor: 3.063
Authors: John A Conti; Timothy J Kendall; Adrian Bateman; Thomas A Armstrong; Andrew Papa-Adams; Qian Xu; Graham Packham; John N Primrose; R Christopher Benyon; John P Iredale Journal: Clin Cancer Res Date: 2008-10-15 Impact factor: 12.531