| Literature DB >> 15619516 |
D Stark1, N Beebe, D Marriott, J Ellis, J Harkness.
Abstract
Dientamoeba fragilis is a trichomonad parasite that causes human gastrointestinal disease. Currently microscopy is considered to be the gold standard for diagnosis of D. fragilis infection. However, this method is time-consuming and relatively insensitive. A PCR assay based on the small-subunit ribosomal RNA gene of D. fragilis for the specific detection of D. fragilis DNA in fresh unpreserved stool samples was developed. The D. fragilis PCR was positive in 29/31 samples with positive microscopy and did not cross-react with other protozoan parasites. The PCR protocol showed a specificity of 100% and a sensitivity of 93.5% and the entire procedure can be performed in one day.Entities:
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Year: 2005 PMID： 15619516 DOI： 10.1016/j.ijpara.2004.09.003
Source DB: PubMed Journal: Int J Parasitol ISSN： 0020-7519 Impact factor: 3.981