BACKGROUND: Recent reports indicate that repeated electroconvulsive shock (ECS) induces cortical cell proliferation, suggesting the possibility that ECS may activate cell cycle progression in the rat brain cortex. METHODS: Sprague-Dawley rats (150-200g) were divided into four treatment groups and then given sham treatment or ECS treatment for 1, 5, and 10 days, respectively. The activity of cyclin-dependent kinase 2 (Cdk2), phosphorylation, and total protein amount of cyclin D1, cyclin E, pocket retinoblastoma family of protein (pRB), and E2F1 were analyzed in the rat cerebral cortex. RESULTS: The activity of Cdk2, the protein amount of pRB, Ser795 phosphorylation of pRB, and the protein amount of E2F1 were all increased compared with the sham-treated control subjects, and these increases were enhanced with the increasing number of ECS. In contrast, the protein amounts of Cdk2, cyclin D1, and cyclin E were not changed by repeated ECS. CONCLUSIONS: The Cdk2-pRB-E2F1 cell cycle pathway is activated by repeated ECS in the rat frontal cortex.
BACKGROUND: Recent reports indicate that repeated electroconvulsive shock (ECS) induces cortical cell proliferation, suggesting the possibility that ECS may activate cell cycle progression in the rat brain cortex. METHODS:Sprague-Dawley rats (150-200g) were divided into four treatment groups and then given sham treatment or ECS treatment for 1, 5, and 10 days, respectively. The activity of cyclin-dependent kinase 2 (Cdk2), phosphorylation, and total protein amount of cyclin D1, cyclin E, pocket retinoblastoma family of protein (pRB), and E2F1 were analyzed in the rat cerebral cortex. RESULTS: The activity of Cdk2, the protein amount of pRB, Ser795 phosphorylation of pRB, and the protein amount of E2F1 were all increased compared with the sham-treated control subjects, and these increases were enhanced with the increasing number of ECS. In contrast, the protein amounts of Cdk2, cyclin D1, and cyclin E were not changed by repeated ECS. CONCLUSIONS: The Cdk2-pRB-E2F1 cell cycle pathway is activated by repeated ECS in the rat frontal cortex.
Authors: Josefine S Witteveen; Marjolein H Willemsen; Thaís C D Dombroski; Nick H M van Bakel; Willy M Nillesen; Josephus A van Hulten; Eric J R Jansen; Dave Verkaik; Hermine E Veenstra-Knol; Conny M A van Ravenswaaij-Arts; Jolien S Klein Wassink-Ruiter; Marie Vincent; Albert David; Cedric Le Caignec; Jolanda Schieving; Christian Gilissen; Nicola Foulds; Patrick Rump; Tim Strom; Kirsten Cremer; Alexander M Zink; Hartmut Engels; Sonja A de Munnik; Jasper E Visser; Han G Brunner; Gerard J M Martens; Rolph Pfundt; Tjitske Kleefstra; Sharon M Kolk Journal: Nat Genet Date: 2016-07-11 Impact factor: 38.330
Authors: Won Je Jeon; Se Hyun Kim; Myoung Suk Seo; Yeni Kim; Ung Gu Kang; Yong-Sung Juhnn; Yong Sik Kim Journal: Exp Mol Med Date: 2008-08-31 Impact factor: 8.718