Literature DB >> 15606637

Determination of Cryptococcus neoformans var. neoformans mating type by multiplex PCR.

M C Esposto1, M Cogliati, A M Tortorano, M A Viviani.   

Abstract

Mating type plays an important role in the epidemiology and virulence of Cryptococcus neoformans. The present study designed a multiplex PCR method to distinguish the six mating type patterns (Aa, Da, Aalpha, Dalpha, Aa/Dalpha, and Aalpha/Da) of C. neoformans var. neoformans. PCR amplification identified one fragment for Aa (860 bp), Dalpha (413 bp) and Da (645 bp) strains, two fragments for Aalpha (320 and 400 bp) and Aa/Dalpha (860 and 413 bp) strains, and three fragments (645, 400, 320 bp) for an Aalpha/Da strain. The method appears to be a valid, simple and relatively inexpensive tool for epidemiological and virulence studies.

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Year:  2004        PMID： 15606637     DOI： 10.1111/j.1469-0691.2004.00972.x

Source DB:  PubMed          Journal:  Clin Microbiol Infect        ISSN： 1198-743X            Impact factor:   8.067


  11 in total

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Journal:  FEMS Yeast Res       Date:  2016-05-05       Impact factor: 2.796

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Authors:  Mohamed S Ellabib; Mohamed A Aboshkiwa; Walid M Husien; Roberta D'Amicis; Massimo Cogliati
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Journal:  Fungal Genet Biol       Date:  2016-01-06       Impact factor: 3.495

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Journal:  PLoS One       Date:  2016-10-20       Impact factor: 3.240

8.  Mortality due to Cryptococcus neoformans and Cryptococcus gattii in low-income settings: an autopsy study.

Authors:  Juan Carlos Hurtado; Paola Castillo; Fabiola Fernandes; Mireia Navarro; Lucilia Lovane; Isaac Casas; Llorenç Quintó; Francesc Marco; Dercio Jordao; Mamudo R Ismail; Cesaltina Lorenzoni; Antonio E Martinez-Palhares; Luiz Ferreira; Marcus Lacerda; Wuelton Monteiro; Ariadna Sanz; Emilio Letang; Lorena Marimon; Susan Jesri; Anelsio Cossa; Inacio Mandomando; Jordi Vila; Quique Bassat; Jaume Ordi; Clara Menéndez; Carla Carrilho; Miguel J Martínez
Journal:  Sci Rep       Date:  2019-05-16       Impact factor: 4.379

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10.  Epidemiological characteristics of cryptococcal meningoencephalitis associated with Cryptococcus neoformans var. grubii from HIV-infected patients in Madagascar: A cross-sectional study.

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Journal:  PLoS Negl Trop Dis       Date:  2020-01-13
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