Literature DB >> 15604827

Real Time RT-PCR and flow cytometry to investigate wheat kernel hardness: role of puroindoline genes and proteins.

M G Amoroso1, L Longobardo, R Capparelli.   

Abstract

Developing seeds from Triticum aestivum (wheat) cultivars were collected after flowering and analysed for puroindoline a and b gene expression by Real Time RT-PCR. Mature seeds were investigated for the presence and the amount of starch-associated puroindoline a and b proteins by flow cytometry. Puroindoline a gene and protein were found to have a predominant role in controlling wheat kernel hardness.

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Year:  2004        PMID： 15604827     DOI： 10.1007/s10529-004-3745-3

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  7 in total

Review 1.  Molecular genetics of puroindolines and related genes: regulation of expression, membrane binding properties and applications.

Authors:  Mrinal Bhave; Craig F Morris
Journal:  Plant Mol Biol       Date:  2007-11-30       Impact factor: 4.076

2.  Two plant puroindolines colocalize in wheat seed and in vitro synergistically fight against pathogens.

Authors:  Rosanna Capparelli; Maria Grazia Amoroso; Daniela Palumbo; Marco Iannaccone; Claudia Faleri; Mauro Cresti
Journal:  Plant Mol Biol       Date:  2005-08       Impact factor: 4.076

3.  Complementation of the pina (null) allele with the wild type Pina sequence restores a soft phenotype in transgenic wheat.

Authors:  J M Martin; F D Meyer; E D Smidansky; H Wanjugi; A E Blechl; M J Giroux
Journal:  Theor Appl Genet       Date:  2006-09-20       Impact factor: 5.699

4.  Transgenic rice plants harboring the grain hardness-locus region of Aegilops tauschii.

Authors:  Go Suzuki; Hideo Wada; Hiromi Goto; Akiko Nakano; Haruna Oba; Takuya Deno; Sadequr Rahman; Yasuhiko Mukai
Journal:  Plant Cell Rep       Date:  2011-08-18       Impact factor: 4.570

Review 5.  The determinants of grain texture in cereals.

Authors:  A Nadolska-Orczyk; S Gasparis; W Orczyk
Journal:  J Appl Genet       Date:  2009       Impact factor: 3.240

6.  Assessment of genetic and epigenetic variation during long-term Taxus cell culture.

Authors:  Chunhua Fu; Liqin Li; Wenjuan Wu; Maoteng Li; Xiaoqing Yu; Longjiang Yu
Journal:  Plant Cell Rep       Date:  2012-05-05       Impact factor: 4.570

7.  Influence of Gene Expression on Hardness in Wheat.

Authors:  Ravi C Nirmal; Agnelo Furtado; Colin Wrigley; Robert J Henry
Journal:  PLoS One       Date:  2016-10-14       Impact factor: 3.240
  7 in total

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