OBJECTIVE: Identification of the presence and drug resistance of Mycobacterium leprae is key to the diagnosis and treatment of leprosy in non-endemic country like Korea. The aim of this study was to screen the drug target DNA such as folP, rpoB, gyr, and 23S rRNA of drug resistance strain of M. leprae. PATIENTS AND METHODS: Sequences of those genes were analyzed for the 104 bacterial index positive cases out of 171 leprosy patients in Korea using touchdown PCR, single stranded conformational polymorphism. RESULTS: Twenty (19.2%) cases have shown the mutations in folP gene of dapsone-resistant M. leprae in which three (2.89%) cases were mutations in two genes, folP and rpoB, of multidrugs resistant strains to dapsone and rifampin, and two (1.92%) cases in folP and gyr genes of resistance to dapsone and oflaxacin, respectively. Besides double mutation for folP gene was one case (0.96%) and for rpoB gene one case, respectively. There was no mutant isolates in 23S rRNA gene against clarithromycin. CONCLUSIONS: This result should leads to a better understanding of the status of multidrug resistant leprosy in Korea and may assist in the rapid diagnosis of drug resistant M. leprae and the choice of the appropriate treatment regimens.
OBJECTIVE: Identification of the presence and drug resistance of Mycobacterium leprae is key to the diagnosis and treatment of leprosy in non-endemic country like Korea. The aim of this study was to screen the drug target DNA such as folP, rpoB, gyr, and 23S rRNA of drug resistance strain of M. leprae. PATIENTS AND METHODS: Sequences of those genes were analyzed for the 104 bacterial index positive cases out of 171 leprosypatients in Korea using touchdown PCR, single stranded conformational polymorphism. RESULTS: Twenty (19.2%) cases have shown the mutations in folP gene of dapsone-resistant M. leprae in which three (2.89%) cases were mutations in two genes, folP and rpoB, of multidrugs resistant strains to dapsone and rifampin, and two (1.92%) cases in folP and gyr genes of resistance to dapsone and oflaxacin, respectively. Besides double mutation for folP gene was one case (0.96%) and for rpoB gene one case, respectively. There was no mutant isolates in 23S rRNA gene against clarithromycin. CONCLUSIONS: This result should leads to a better understanding of the status of multidrug resistant leprosy in Korea and may assist in the rapid diagnosis of drug resistant M. leprae and the choice of the appropriate treatment regimens.
Authors: Pushpendra Singh; Philippe Busso; Alberto Paniz-Mondolfi; Nacarid Aranzazu; Marc Monot; Nadine Honore; Andrea de Faria Fernandes Belone; Marcos Virmond; Maria Esther Villarreal-Olaya; Carlos Rivas; Stewart T Cole Journal: Antimicrob Agents Chemother Date: 2011-03-28 Impact factor: 5.191
Authors: Adalgiza da Silva Rocha; Alexandre Araujo Cunha Dos Santos; Patrícia Pignataro; José Augusto Nery; Antônio Basílio de Miranda; Diego Fonseca Soares; Amanda Nogueira Brum Fontes; Alice Miranda; Helen Ferreira; Neio Boéchat; Maria Eugênia Novisck Gallo; Euzenir Nunes Sarno; Maria Leide W De Oliveira; Philip Noel Suffys Journal: J Med Microbiol Date: 2011-05-19 Impact factor: 2.472
Authors: Adalgiza da Silva Rocha; Maria das Graças Cunha; Lucia Martins Diniz; Claudio Salgado; Maria Araci P Aires; José Augusto Nery; Eugênia Novisck Gallo; Alice Miranda; Monica M F Magnanini; Masanori Matsuoka; Euzenir Nunes Sarno; Philip Noel Suffys; Maria Leide W de Oliveira Journal: J Clin Microbiol Date: 2012-04-11 Impact factor: 5.948