| Literature DB >> 15601667 |
Dagmar Eleveld-Trancikova1, Vassilis Triantis, Veronique Moulin, Maaike W G Looman, Mietske Wijers, Jack A M Fransen, Angelique A C Lemckert, Menzo J E Havenga, Carl G Figdor, Richard A J Janssen, Gosse J Adema.
Abstract
Recently, we described the molecular identification of dendritic cell-specific TrAnsMembrane protein (DC-STAMP), a multimembrane-spanning protein preferentially expressed by human DC (hDC). In this report, we describe the identification and expression profile of the murine homologue of DC-STAMP (mDC-STAMP) as well as the characterization of the DC-STAMP protein. The results demonstrate that mDC-STAMP is over 90% homologous to hDC-STAMP and is also preferentially expressed by DC in vitro and ex vivo. mDC-STAMP expression is enhanced by interleukin-4 and down-regulated upon DC maturation. Analysis of differently tagged DC-STAMP proteins further demonstrates that hDC-STAMP and mDC-STAMP are glycosylated and primarily localize to an intracellular compartment. Applying confocal microscopy and electron microscopy, we demonstrate that hDC-STAMP localizes to the endoplasmic reticulum (ER) in human embryonic kidney 293 cells as well as hDC transduced with an adenovirus encoding hDC-STAMP-green fluorescent protein fusion protein. These data imply that DC-STAMP may exert its effect in the ER.Entities:
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Year: 2004 PMID： 15601667 DOI： 10.1189/jlb.0804441
Source DB: PubMed Journal: J Leukoc Biol ISSN： 0741-5400 Impact factor: 4.962