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Literature DB >> 1559842

Immunohistochemical demonstration of a new thiamine diphosphate-binding protein in the rat digestive tract.

H Yoshioka¹, K Nishino, T Usui, M Sung, G Ohshio, T Sugiyama, T Kita.

Abstract

We purified a new thiamine diphosphate-binding protein (ThDP-BP), produced an antiserum against it, and examined its immunohistochemical distribution in the rat gastrointestinal tract using the avidin-biotin complex method. Positive staining for ThDP-BP was found in the epithelial glands of the stomach, small intestine and large intestine, and in the nuclei of hepatocytes. Measurement of the content of thiamine and its phosphate esters in extracts from the gastric and intestinal mucosa also indicated the presence of ThDP-BP in the stomach and intestinal mucosa. ThDP-BP may be useful for investigating the absorption and metabolism of the thiamine in metabolic disorders.

Entities: Chemical Disease Species

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Year: 1992 PMID： 1559842 DOI： 10.1007/bf00267301

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

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References

19 in total

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Immunohistochemical demonstration of a new thiamine diphosphate-binding protein in the rat digestive tract.

1. Thiamine and nerve membrane.

2. On the etiology of the alcoholic neurologic diseases with special reference to the role of nutrition.

3. The action of thiamine and its di- and triphosphates on the slow exponential decline of the ionic currents in the node of Ranvier.

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10. Thiamine and cholinergic transmission in the electric organ of Torpedo. I. Cellular localization and functional changes of thiamine and thiamine phosphate esters.