| Literature DB >> 15596892 |
Abstract
Horseradish peroxidase is coupled to IgG antibody in a two-step procedure. In the first step monosaccharide residues in the enzyme are oxidized with periodate to produce aldehyde groups. Then, in the second step, the aldehyde groups are allowed to react with amino groups in the IgG antibody. The Schiff bases formed are reduced and the conjugate is purified by gel filtration.Entities:
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Year: 2005 PMID： 15596892 DOI： 10.1385/1-59259-873-0:127
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745