OBJECTIVE: To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin. METHODS: The recombinant plasmid pGEMEX-1-Humanin was digested with restriction endonucleases BamH I and Hind III and the Humanin gene fragments, about 100 bp length, were obtained. Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-) and the recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing. RESULTS: Recombinant plasmid DNA successfully produced a band which had the same size as that of the Humanin positive control. The sequence of recombinant plasmids accorded with the Humnain gene sequence. CONCLUSIONS: A eukaryotic expression plasmid of Humanin was successfully constructed.
OBJECTIVE: To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin. METHODS: The recombinant plasmid pGEMEX-1-Humanin was digested with restriction endonucleases BamH I and Hind III and the Humanin gene fragments, about 100 bp length, were obtained. Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-) and the recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing. RESULTS: Recombinant plasmid DNA successfully produced a band which had the same size as that of the Humanin positive control. The sequence of recombinant plasmids accorded with the Humnain gene sequence. CONCLUSIONS: A eukaryotic expression plasmid of Humanin was successfully constructed.
Authors: Y Hashimoto; T Niikura; H Tajima; T Yasukawa; H Sudo; Y Ito; Y Kita; M Kawasumi; K Kouyama; M Doyu; G Sobue; T Koide; S Tsuji; J Lang; K Kurokawa; I Nishimoto Journal: Proc Natl Acad Sci U S A Date: 2001-05-22 Impact factor: 11.205
Authors: Y Hashimoto; T Niikura; Y Ito; H Sudo; M Hata; E Arakawa; Y Abe; Y Kita; I Nishimoto Journal: J Neurosci Date: 2001-12-01 Impact factor: 6.167