Literature DB >> 15592613

NMR conformational analysis of biosynthetic precursor-type lipid A: monomolecular state and supramolecular assembly.

Masato Oikawa1, Tetsuya Shintaku, Naohiro Fukuda, Harald Sekljic, Yoshiyuki Fukase, Hiroaki Yoshizaki, Koichi Fukase, Shoichi Kusumoto.   

Abstract

The detailed conformational analysis of a single molecule of the tetraacyl biosynthetic precursor-type lipid A and its characteristic supramolecular assembly in aqueous SDS-micelles are described. Regular molecular arrangements were observed by detailed analysis of the NMR spectra of synthetically pure specimens, including regiospecifically 13C-labeled ones. NMR analysis of a biologically inactive precursor-type analogue with four shorter acyl chains demonstrated its conformational flexibility, indicating the importance of hydrophobic interactions for maintaining the conformation of such molecules.

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Year:  2004        PMID： 15592613     DOI： 10.1039/b410544c

Source DB:  PubMed          Journal:  Org Biomol Chem        ISSN： 1477-0520            Impact factor:   3.876


  5 in total

1.  Activation of Human Toll-like Receptor 4 (TLR4)·Myeloid Differentiation Factor 2 (MD-2) by Hypoacylated Lipopolysaccharide from a Clinical Isolate of Burkholderia cenocepacia.

Authors:  Flaviana Di Lorenzo; Łukasz Kubik; Alja Oblak; Nicola Ivan Lorè; Cristina Cigana; Rosa Lanzetta; Michelangelo Parrilli; Mohamad A Hamad; Anthony De Soyza; Alba Silipo; Roman Jerala; Alessandra Bragonzi; Miguel A Valvano; Sonsoles Martín-Santamaría; Antonio Molinaro
Journal:  J Biol Chem       Date:  2015-07-09       Impact factor: 5.157

2.  Interaction of lipopolysaccharide and phospholipid in mixed membranes: solid-state 31P-NMR spectroscopic and microscopic investigations.

Authors:  Kaoru Nomura; Takehiko Inaba; Kenichi Morigaki; Klaus Brandenburg; Ulrich Seydel; Shoichi Kusumoto
Journal:  Biophys J       Date:  2008-05-02       Impact factor: 4.033

3.  Anti-endotoxic activity and structural basis for human MD-2·TLR4 antagonism of tetraacylated lipid A mimetics based on βGlcN(1↔1)αGlcN scaffold.

Authors:  Jose Antonio Garate; Johannes Stöckl; María del Carmen Fernández-Alonso; Daniel Artner; Mira Haegman; Chris Oostenbrink; Jesús Jiménez-Barbero; Rudi Beyaert; Holger Heine; Paul Kosma; Alla Zamyatina
Journal:  Innate Immun       Date:  2014-11-13       Impact factor: 2.680

4.  Conformationally constrained lipid A mimetics for exploration of structural basis of TLR4/MD-2 activation by lipopolysaccharide.

Authors:  Daniel Artner; Alja Oblak; Simon Ittig; Jose Antonio Garate; Simon Horvat; Cécile Arrieumerlou; Andreas Hofinger; Chris Oostenbrink; Roman Jerala; Paul Kosma; Alla Zamyatina
Journal:  ACS Chem Biol       Date:  2013-09-26       Impact factor: 5.100

5.  Insights into the species-specific TLR4 signaling mechanism in response to Rhodobacter sphaeroides lipid A detection.

Authors:  Muhammad Ayaz Anwar; Suresh Panneerselvam; Masaud Shah; Sangdun Choi
Journal:  Sci Rep       Date:  2015-01-07       Impact factor: 4.379
  5 in total

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