OBJECTIVE: CD1d-restricted natural killer T (NKT) cells are reported to play a proatherogenic role in the development of atherosclerosis. However, the contribution of NKT cells to mature lesion formation and the effector mechanisms through which they act are unknown. METHODS AND RESULTS: We measured lesion size in CD1d-null (CD1d-/-) mice on the low-density lipoprotein (LDL) receptor-deficient (LDLR-/-) genetic background after 4, 8, and 12 weeks of feeding on a Western diet. Lesions in CD1d-/-LDLR-/- mice were 47% smaller at 4 weeks than CD1d+/+LDLR-/- controls; however, there were no differences in lesion size between CD1d-/-LDLR-/- and CD1d+/+LDLR-/- mice at 8 or 12 weeks. We found that although NKT cells were present in the aortic arch of CD1d+/+LDLR-/- mice on the Western diet, no differences in mRNA abundance for Th1 or Th2 cytokines were observed between CD1d-/-LDLR-/- and CD1d+/+LDLR-/- mice. CONCLUSIONS: CD1d-restricted NKT cells contribute to the formation of fatty streaks; however, their influence on lesion progression is transient, and they do not significantly affect the inflammatory cytokine milieu of mature lesions.
OBJECTIVE:CD1d-restricted natural killer T (NKT) cells are reported to play a proatherogenic role in the development of atherosclerosis. However, the contribution of NKT cells to mature lesion formation and the effector mechanisms through which they act are unknown. METHODS AND RESULTS: We measured lesion size in CD1d-null (CD1d-/-) mice on the low-density lipoprotein (LDL) receptor-deficient (LDLR-/-) genetic background after 4, 8, and 12 weeks of feeding on a Western diet. Lesions in CD1d-/-LDLR-/- mice were 47% smaller at 4 weeks than CD1d+/+LDLR-/- controls; however, there were no differences in lesion size between CD1d-/-LDLR-/- and CD1d+/+LDLR-/- mice at 8 or 12 weeks. We found that although NKT cells were present in the aortic arch of CD1d+/+LDLR-/- mice on the Western diet, no differences in mRNA abundance for Th1 or Th2 cytokines were observed between CD1d-/-LDLR-/- and CD1d+/+LDLR-/- mice. CONCLUSIONS:CD1d-restricted NKT cells contribute to the formation of fatty streaks; however, their influence on lesion progression is transient, and they do not significantly affect the inflammatory cytokine milieu of mature lesions.
Authors: Laura E Felley; Akshat Sharma; Erin Theisen; James C Romero-Masters; John-Demian Sauer; Jenny E Gumperz Journal: J Immunol Date: 2016-08-17 Impact factor: 5.422
Authors: Sreya Bagchi; Ying He; Hong Zhang; Liang Cao; Ildiko Van Rhijn; D Branch Moody; Johann E Gudjonsson; Chyung-Ru Wang Journal: J Clin Invest Date: 2017-05-02 Impact factor: 14.808
Authors: Duygu Sag; Gerhard Wingender; Heba Nowyhed; Runpei Wu; Abraham K Gebre; John S Parks; Mitchell Kronenberg; Catherine C Hedrick Journal: J Immunol Date: 2012-10-24 Impact factor: 5.422