Literature DB >> 15585914

Generation of clinical-grade monocyte-derived dendritic cells using the CliniMACS system.

Thomas Putz1, Hubert Gander, Reinhold Ramoner, Claudia Zelle-Rieser, Andrea Rahm, Walter Nussbaumer, Georg Bartsch, Lorenz Höltl, Martin Thurnher.   

Abstract

We describe the generation of monocyte-derived dendritic cells (MoDC) from leukapheresis products using the CliniMACS system from Miltenyi Biotec. In a clinical setting, the method turned out to be feasible for the generation of clinical-grade MoDC from patients with metastatic renal-cell carcinoma. MoDC generated with this system exhibited a fully mature phenotype as well as high migratory and T-cell stimulatory capacity.

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Year:  2005        PMID： 15585914     DOI： 10.1385/1-59259-862-5:071

Source DB:  PubMed          Journal:  Methods Mol Med        ISSN： 1543-1894


  2 in total

1.  Monitoring of CD4+ and CD8+ T-cell responses after dendritic cell-based immunotherapy using CFSE dye dilution analysis.

Authors:  Thomas Putz; Reinhold Ramoner; Hubert Gander; Andrea Rahm; Georg Bartsch; Lorenz Höltl; Martin Thurnher
Journal:  J Clin Immunol       Date:  2004-11       Impact factor: 8.317

2.  Counter-flow elutriation of clinical peripheral blood mononuclear cell concentrates for the production of dendritic and T cell therapies.

Authors:  David F Stroncek; Vicki Fellowes; Chauha Pham; Hanh Khuu; Daniel H Fowler; Lauren V Wood; Marianna Sabatino
Journal:  J Transl Med       Date:  2014-09-17       Impact factor: 5.531
  2 in total

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