Literature DB >> 15585305

Non-invasive live-cell measurement of changes in macrophage NAD(P)H by two-photon microscopy.

Eleanor P W Kable1, Alexandra K Kiemer.   

Abstract

Two-photon microscopy allows determination of UV-excitable fluorophores using long-wavelength light. We aimed to determine NAD(P)H autofluorescence as a measure for macrophage NADPH-oxidase activation. RAW264.7 macrophages were grown on glass coverslips and kept in HBSS for microscopic investigation. Cells were excited with 710 nm light and NAD(P)H autofluorescence was detected. Glucose as well as NaCN evoked an increase of NAD(P)H autofluorescence. Activators of NADPH oxidase lead to significantly decreased NAD(P)H autofluorescence. Therefore, this work shows the suitability of two-photon microscopy as a non-invasive method determining changes in phagocyte NAD(P)H upon activation.

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Year:  2005        PMID： 15585305     DOI： 10.1016/j.imlet.2003.12.013

Source DB:  PubMed          Journal:  Immunol Lett        ISSN： 0165-2478            Impact factor:   3.685


  6 in total

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Authors:  Nicolas Pavillon; Alison J Hobro; Shizuo Akira; Nicholas I Smith
Journal:  Proc Natl Acad Sci U S A       Date:  2018-03-06       Impact factor: 11.205

Review 2.  Intracellular coenzymes as natural biomarkers for metabolic activities and mitochondrial anomalies.

Authors:  Ahmed A Heikal
Journal:  Biomark Med       Date:  2010-04       Impact factor: 2.851

3.  Two-photon autofluorescence dynamics imaging reveals sensitivity of intracellular NADH concentration and conformation to cell physiology at the single-cell level.

Authors:  Qianru Yu; Ahmed A Heikal
Journal:  J Photochem Photobiol B       Date:  2008-12-25       Impact factor: 6.252

4.  Two-photon fluorescence lifetime imaging monitors metabolic changes during wound healing of corneal epithelial cells in vitro.

Authors:  Uta Gehlsen; Andrea Oetke; Márta Szaszák; Norbert Koop; Friedrich Paulsen; Andreas Gebert; Gereon Huettmann; Philipp Steven
Journal:  Graefes Arch Clin Exp Ophthalmol       Date:  2012-05-06       Impact factor: 3.117

5.  From morphology to biochemical state - intravital multiphoton fluorescence lifetime imaging of inflamed human skin.

Authors:  Volker Huck; Christian Gorzelanny; Kai Thomas; Valentina Getova; Verena Niemeyer; Katharina Zens; Tim R Unnerstall; Julia S Feger; Mohammad A Fallah; Dieter Metze; Sonja Ständer; Thomas A Luger; Karsten Koenig; Christian Mess; Stefan W Schneider
Journal:  Sci Rep       Date:  2016-03-23       Impact factor: 4.379

6.  NO-dependent attenuation of TPA-induced immunoinflammatory skin changes in Balb/c mice by pindolol, heptaminol or ATRA, but not by verapamil.

Authors:  Jinhyuk F Chung; Calvin J Yoon; Seon Ah Cheon; Eun Seok Seo; Sung Ho Park; Jae Seung Yang; Bumju Kim; Min Young Joo; Tae Jung Park; Ki Hean Kim; Anil K Sood; Sang Joon Lee
Journal:  Oncotarget       Date:  2016-07-26
  6 in total

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