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Literature DB >> 15585293

Quantitative RT-PCR assay of 5-HT1A and 5-HT2A serotonin receptor mRNAs using genomic DNA as an external standard.

Alexander V Kulikov¹, Vladimir S Naumenko, Irina P Voronova, Maria A Tikhonova, Nina K Popova.

Abstract

Brain serotonin 5-HT(1A) and 5-HT(2A) receptors have been implicated in both normal and pathological behavior, and in the action of anxiolytic and antidepressant drugs. In this study, detailed description and verification of a new RT-PCR technique to quantify the number of copies of 5-HT(1A) and 5-HT(2A) receptor mRNAs in the brain is presented. The number of copies of beta-actin and 5-HT(1A) or 5-HT(2A) receptor mRNAs in rat brain samples was evaluated with respect to the genomic DNA solution as the external exogenous standard. The expression of 5-HT receptors was calculated as the number of receptor mRNA copies per 100 copies of corresponding beta-actin mRNA. This presented technique is reliable, simple and can be easily set up in any neurobiological laboratory.

Entities: Gene Species

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Year: 2005 PMID： 15585293 DOI： 10.1016/j.jneumeth.2004.06.005

Source DB: PubMed Journal: J Neurosci Methods ISSN： 0165-0270 Impact factor: 2.390

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Cited

13 in total

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Journal: Mol Neurobiol Date: 2014-08-02 Impact factor: 5.590

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5. Chronic administration of imipramine normalizes decreased sexual motivation and increased predisposition to catalepsy induced by propylthiouracil in rats.

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6. Involvement of brain serotonin 5-HT1A receptors in genetic predisposition to aggressive behavior.

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Journal: Br J Pharmacol Date: 2016-04-21 Impact factor: 8.739