Literature DB >> 1557829

A simple technique for postembedding ultrastructural immunogold labeling.

V G Lockard1.   

Abstract

A simple and reproducible method for postembedding ultrastructural immunogold labeling is described. Tissues are fixed in Carson-Millonig phosphate-buffered 4% formaldehyde, and then are embedded in LR White resin. Immunogold labeling of ultrathin sections is accomplished by means of an indirect method that employs a nonconjugated primary antibody and gold particles conjugated to antibody to immunoglobulin G. Technical aspects of fixation, embedding, and immunolabeling that influence the success of this protocol are discussed.

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Year:  1992        PMID： 1557829     DOI： 10.3109/01913129209074549

Source DB:  PubMed          Journal:  Ultrastruct Pathol        ISSN： 0191-3123            Impact factor:   1.094


  2 in total

1.  Defects in nuclear structure and function promote dilated cardiomyopathy in lamin A/C-deficient mice.

Authors:  Vesna Nikolova; Christiana Leimena; Aisling C McMahon; Ju Chiat Tan; Suchitra Chandar; Dilesh Jogia; Scott H Kesteven; Jan Michalicek; Robyn Otway; Fons Verheyen; Stephen Rainer; Colin L Stewart; David Martin; Michael P Feneley; Diane Fatkin
Journal:  J Clin Invest       Date:  2004-02       Impact factor: 14.808

2.  Conserved Role of the Large Conductance Calcium-Activated Potassium Channel, KCa1.1, in Sinus Node Function and Arrhythmia Risk.

Authors:  Santiago Pineda; Vesna Nikolova-Krstevski; Christiana Leimena; Karen Ocorr; Diane Fatkin; Andrew J Atkinson; Ann-Kristin Altekoester; Charles D Cox; Arie Jacoby; Inken G Huttner; Yue-Kun Ju; Magdalena Soka; Monique Ohanian; Gunjan Trivedi; Sreehari Kalvakuri; Katja Birker; Renee Johnson; Peter Molenaar; Dennis Kuchar; David G Allen; Dirk F van Helden; Richard P Harvey; Adam P Hill; Rolf Bodmer; Georg Vogler; Halina Dobrzynski
Journal:  Circ Genom Precis Med       Date:  2021-02-25
  2 in total

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