L-aspartate-immunoreactive neurons in the rat enteric nervous system.

L-aspartate (L-Asp) is an excitatory neurotransmitter in the central nervous system. In the present study, we demonstrate, for the first time, the presence of L-Asp in a particular neuronal cell class in the enteric nervous system (ENS). Scattered L-Asp-immunoreactive neuronal cell bodies and nerve fibers were found extensively in both the myenteric and submucosal plexus throughout the small and large intestines. Many L-Asp-immunoreactive nerve fibers, which originated from intrinsic nerve cell bodies, were found in the ganglia and interconnecting nerve bundles. Electron microscopy revealed that L-Asp-immunoreactive terminals frequently formed synaptic contacts with intrinsic nerve cells, suggesting that some L-Asp-immunoreactive neurons might function as interneurons. These results suggest that L-Asp-immunoreactive neurons play a significant role within the ENS to control intestinal functions. The presence of enteric L-Asp-immunoreactive neurons provides strong support for the proposal that L-Asp is a neuromodulator in the rat ENS.