| Literature DB >> 15576187 |
Xuefei Zhang1, Yuxin Li, Xuesi Chen, Xiuhong Wang, Xiaoyi Xu, Qizhi Liang, Junli Hu, Xiabin Jing.
Abstract
A paclitaxel/MPEG-PLA block copolymer conjugate was prepared in three steps: (1) hydroxyl-terminated diblock copolymer of monomethoxy-poly(ethylene glycol)-b-poly(lactide) (MPEG-PLA) was synthesized by ring-opening polymerization of L-lactide using MPEG as a maroinitiator; (2) it was converted to carboxyl-terminated MPEG-PLA by reacting with mono-t-butyl ester of diglycolic acid and subsequent deprotecting the t-butyl group with TFA; (3) the latter was reacted with paclitaxel in the presence of dicyclohexylcarbodiimide and dimethylaminopyridine. Structures of the polymers synthesized were confirmed by (1)H NMR, and their molecular weights were determined by gel permeation chromatography. The antitumor activity of the conjugate against human liver cancer H7402 cells was evaluated by MTT method. The results showed that paclitaxel can be released from the conjugate without losing cytotoxicity.Entities:
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Year: 2005 PMID： 15576187 DOI： 10.1016/j.biomaterials.2004.06.024
Source DB: PubMed Journal: Biomaterials ISSN： 0142-9612 Impact factor: 12.479