| Literature DB >> 1556922 |
T J Mosher1, G D Williams, C Doumen, K F LaNoue, M B Smith.
Abstract
The measurement of free intracellular magnesium (Mg2+) using the 31P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemical-shift limits delta ATP alpha beta and delta MgATP alpha beta. Solutions containing excess Mg2+ contain significant amounts of Mg2ATP and yield positive errors in the value of delta MgATP alpha beta. For physiological applications this may overestimate free intracellular Mg2+ by as much as 300%. This error may be minimized if appropriate mole ratios of Mg2+/ATP are used to calibrate delta MgATP alpha beta.Entities:
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Year: 1992 PMID： 1556922 DOI： 10.1002/mrm.1910240117
Source DB: PubMed Journal: Magn Reson Med ISSN： 0740-3194 Impact factor: 4.668