A D Kitchen1, P H J Lowe, K Lalloo, P L Chiodini. 1. National Transfusion Microbiology Reference Laboratory, National Blood Service, London, UK. alan.kitchen@nbs.nhs.uk
Abstract
BACKGROUND AND OBJECTIVES: A new recombinant Plasmodium antigen enzyme immunoassay (EIA) for the detection of malarial antibodies was evaluated for the screening of 'malaria-risk' blood and tissue donations. MATERIALS AND METHODS: A total of 13,269 donor and patient samples were tested by both the EIA and the standard diagnostic antibody immunofluorescence test (IFAT). RESULTS: A total of 114/138 (82.6%) samples from patients with P. falciparum and 11/13 (84.6%) samples from patients with P. vivax tested positive. A total of 714/13,053 (5.47%) samples from donors identified as 'malaria risk', owing to residency or travel, were reactive in the EIA. CONCLUSIONS: The assay is more sensitive than a previously implemented malarial antibody EIA (73% in acute P. falciparum and 56% in acute P. vivax infections). The sensitivity of this new EIA is comparable to that of the IFAT, and the specificity is sufficient to screen 'malaria-risk' donors.
BACKGROUND AND OBJECTIVES: A new recombinant Plasmodium antigen enzyme immunoassay (EIA) for the detection of malarial antibodies was evaluated for the screening of 'malaria-risk' blood and tissue donations. MATERIALS AND METHODS: A total of 13,269 donor and patient samples were tested by both the EIA and the standard diagnostic antibody immunofluorescence test (IFAT). RESULTS: A total of 114/138 (82.6%) samples from patients with P. falciparum and 11/13 (84.6%) samples from patients with P. vivax tested positive. A total of 714/13,053 (5.47%) samples from donors identified as 'malaria risk', owing to residency or travel, were reactive in the EIA. CONCLUSIONS: The assay is more sensitive than a previously implemented malarial antibody EIA (73% in acute P. falciparum and 56% in acute P. vivaxinfections). The sensitivity of this new EIA is comparable to that of the IFAT, and the specificity is sufficient to screen 'malaria-risk' donors.
Authors: A Scott Muerhoff; Larry G Birkenmeyer; Ruthie Coffey; Bruce J Dille; John W Barnwell; William E Collins; Joann S Sullivan; George J Dawson; Suresh M Desai Journal: Clin Vaccine Immunol Date: 2010-08-11
Authors: Sheila F O'Brien; Sheila Ward; Pierre Gallian; Cécile Fabra; Josiane Pillonel; Alan D Kitchen; Katy Davison; Clive R Seed; Gilles Delage; Whitney R Steele; David A Leiby Journal: Blood Transfus Date: 2019-02-06 Impact factor: 3.443
Authors: Christoph Niederhauser; Caroline Tinguely; Jens Dreier; Tanja Vollmer; Hans Peter Marti; Beatrice Nickel; Julia Maria Klemens; Jens Miguel Warnecke; Peter Gowland Journal: Transfus Med Hemother Date: 2021-05-26 Impact factor: 3.747