Zhi-fu Han1, Ding-ding Shao, Heng Wang. 1. Molecular Parasitology Laboratory, Department of Etiology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Abstract
OBJECTIVE: To clone and express a homologue of human macrophage migration inhibitory factor (MIF) from P. falciparum 3D7--PfMIF. METHODS: The nucleotide sequence of PfMIF was found through blast P. falciparum genomic sequence databases with the amino acid sequence of human MIF (HuMIF). RT-PCR, DNA sequencing, and bioinformatics analysis were used for the cloning of Pfmif gene. The recombinant protein was expressed in E. coli and purified through the affinity column. RESULTS: The full length of Pfmif gene was cloned and sequenced. It was composed of 351 nucleotides and encoded 116 amino acids with the typical characteristic of MIF family. The recombinant protein was successfully expressed and purified. CONCLUSIONS: The Pfmif gene and recombinant protein were successfully isolated and PfMIF was preliminarily identified as a novel member of MIF family.
OBJECTIVE: To clone and express a homologue of humanmacrophage migration inhibitory factor (MIF) from P. falciparum 3D7--PfMIF. METHODS: The nucleotide sequence of PfMIF was found through blast P. falciparum genomic sequence databases with the amino acid sequence of humanMIF (HuMIF). RT-PCR, DNA sequencing, and bioinformatics analysis were used for the cloning of Pfmif gene. The recombinant protein was expressed in E. coli and purified through the affinity column. RESULTS: The full length of Pfmif gene was cloned and sequenced. It was composed of 351 nucleotides and encoded 116 amino acids with the typical characteristic of MIF family. The recombinant protein was successfully expressed and purified. CONCLUSIONS: The Pfmif gene and recombinant protein were successfully isolated and PfMIF was preliminarily identified as a novel member of MIF family.