Literature DB >> 1556092

Purification of the DNA-dependent RNA polymerase from the myxobacterium Stigmatella aurantiaca.

M Heidelbach1, H Skladny, H U Schairer.   

Abstract

The DNA-dependent RNA polymerase (EC 2.7.7.6) of the myxobacterium Stigmatella aurantiaca has been purified. It shows three main polypeptide bands with apparent molecular weights of 146,000, 105,000, and 40,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. beta and beta' subunits of the S. aurantiaca polymerase were shown to migrate in the 146,000-molecular-weight polypeptide band and the main sigma factor was shown to migrate in the 105,000-molecular-weight band by using heterologous antisera.

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Year:  1992        PMID： 1556092      PMCID： PMC205917          DOI： 10.1128/jb.174.8.2733-2735.1992

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  12 in total

1.  Rapid isolation of highly active RNA polymerase from Escherichia coli and its subunits by matrix-bound heparin.

Authors:  H Sternbach; R Engelhardt; A G Lezius
Journal:  Eur J Biochem       Date:  1975-12-01

2.  Cloning and DNA sequence of the gene coding for the major sigma factor from Myxococcus xanthus.

Authors:  S Inouye
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

Review 3.  Structure and function of bacterial sigma factors.

Authors:  J D Helmann; M J Chamberlin
Journal:  Annu Rev Biochem       Date:  1988       Impact factor: 23.643

4.  Characterization of foot-and-mouth disease virus gene products with antisera against bacterially synthesized fusion proteins.

Authors:  K Strebel; E Beck; K Strohmaier; H Schaller
Journal:  J Virol       Date:  1986-03       Impact factor: 5.103

5.  Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases.

Authors:  L A Allison; M Moyle; M Shales; C J Ingles
Journal:  Cell       Date:  1985-09       Impact factor: 41.582

6.  The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit.

Authors:  G S Monastyrskaya; V V Gubanov; S O Guryev; I S Salomatina; T M Shuvaeva; V M Lipkin; E D Sverdlov
Journal:  Nucleic Acids Res       Date:  1982-07-10       Impact factor: 16.971

7.  RNA polymerase II of Drosophila. Relation of its 140,000 Mr subunit to the beta subunit of Escherichia coli RNA polymerase.

Authors:  D Falkenburg; B Dworniczak; D M Faust; E K Bautz
Journal:  J Mol Biol       Date:  1987-06-20       Impact factor: 5.469

8.  Cloning and characterization of RNA polymerase core subunits of Chlamydia trachomatis by using the polymerase chain reaction.

Authors:  J N Engel; J Pollack; F Malik; D Ganem
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

9.  Development-specific sigma-factor essential for late-stage differentiation of Myxococcus xanthus.

Authors:  D Apelian; S Inouye
Journal:  Genes Dev       Date:  1990-08       Impact factor: 11.361

10.  RNA polymerase of Myxococcus xanthus: purification and selective transcription in vitro with bacteriophage templates.

Authors:  K E Rudd; D R Zusman
Journal:  J Bacteriol       Date:  1982-07       Impact factor: 3.490
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