| Literature DB >> 15556710 |
Anthony P Moran1, Bethlehem Shiberu, John A Ferris, Yuriy A Knirel, Sof'ya N Senchenkova, Andreij V Perepelov, Per-Erik Jansson, Joanna B Goldberg.
Abstract
The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 26695 were generated. The LPS of mutant 26695::HP0159Kan did not express either Lewis epitope as detected by immunoblotting, whereas the control strain and 26695::HP1416Kan produced both epitopes. Structural analysis of the LPS of the mutants showed that HP0159 encodes an alpha(1,2/3)-glucosyltransferase whereas HP1416 encodes an alpha(1,2/4)-glucosyltransferase.Entities:
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Year: 2004 PMID： 15556710 DOI： 10.1016/j.femsle.2004.10.004
Source DB: PubMed Journal: FEMS Microbiol Lett ISSN： 0378-1097 Impact factor: 2.742