Literature DB >> 15556523

Two step procedure for purification of enzymatically active prostate-specific antigen from seminal plasma.

B Bindukumar1, Elzbieta Kawinski, Craig Cherrin, Leah M Gambino, Madhavan P N Nair, Stanley A Schwartz, Kailash C Chadha.   

Abstract

The role of prostate-specific antigen (PSA) during the onset of prostate cancer and subsequent tumor growth and metastasis is not well understood. We have developed a simple two step procedure, based on principles of hydrophobic charge-induction chromatography and molecular size chromatography to provide pure free-PSA (f-PSA) preparation that is free from all other known PSA complexes as well as human kallikrein 2 (hK2). The overall recovery of f-PSA is 72%. The isolated f-PSA consists of three known isoforms that corresponds to pI of 6.2, 6.4 and 7.2. f-PSA is enzymatically active and its enzymatic activity can be effectively neutralized by a serine protease inhibitor.

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Year:  2004        PMID： 15556523     DOI： 10.1016/j.jchromb.2004.09.014

Source DB:  PubMed          Journal:  J Chromatogr B Analyt Technol Biomed Life Sci        ISSN： 1570-0232            Impact factor:   3.205


  4 in total

1.  Enzymatic activity of free-prostate-specific antigen (f-PSA) is not required for some of its physiological activities.

Authors:  Kailash C Chadha; Bindukumar B Nair; Srikant Chakravarthi; Rita Zhou; Alejandro Godoy; James L Mohler; Ravikumar Aalinkeel; Stanley A Schwartz; Gary J Smith
Journal:  Prostate       Date:  2011-03-28       Impact factor: 4.104

2.  Human seminal proteinase and prostate-specific antigen are the same protein.

Authors:  Abdul Waheed; Md Imtaiyaz Hassan; Robert L Van Etten; Faizan Ahmad
Journal:  J Biosci       Date:  2008-06       Impact factor: 1.826

3.  Thiophilic-interaction chromatography of enzymatically active tissue prostate-specific antigen (T-PSA) and its modulation by zinc ions.

Authors:  A K Satheesh Babu; M A Vijayalakshmi; Gary J Smith; Kailash C Chadha
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2007-12-04       Impact factor: 3.205

4.  Prostate-specific antigen modulates the expression of genes involved in prostate tumor growth.

Authors:  B Bindukumar; Stanley A Schwartz; Madhavan P N Nair; Ravikumar Aalinkeel; Elzbieta Kawinski; Kailash C Chadha
Journal:  Neoplasia       Date:  2005-03       Impact factor: 5.715
  4 in total

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