AIMS/HYPOTHESIS: Recently an individual variability in the relationships between mean blood glucose levels and HbA1c has been observed among diabetic patients. The aim of this study was to provide an accurate description and evaluation of glycated and glyco-oxidated globins from diabetic subjects and their relationship with HbA1c and plasma glucose values. METHODS: We studied 20 type 2 diabetic and 10 healthy subjects. Plasma samples were analysed by matrix-assisted laser desorption ionisation mass spectrometry. RESULTS: The presence of glycated and glyco-oxidated species of both alpha and beta globin was demonstrated. Values for these showed a good linear relationship with HbA1c values and the mean daily plasma glucose values for the 6 weeks preceding the investigation. Trends differed according to whether patients had chronic complications or not, differences being seen in the slopes of the plots relating HbA1c to the abundance of glycated and glyco-oxidated species. CONCLUSIONS/ INTERPRETATION: The data obtained are consistent with the concept that individuals have a different individual proclivity for oxidation and/or that different oxidation kinetics are related to behavioural and environmental factors. Our data are thus relevant to the analysis of phenotype differences in diabetic patients.
AIMS/HYPOTHESIS: Recently an individual variability in the relationships between mean blood glucose levels and HbA1c has been observed among diabeticpatients. The aim of this study was to provide an accurate description and evaluation of glycated and glyco-oxidated globins from diabetic subjects and their relationship with HbA1c and plasma glucose values. METHODS: We studied 20 type 2 diabetic and 10 healthy subjects. Plasma samples were analysed by matrix-assisted laser desorption ionisation mass spectrometry. RESULTS: The presence of glycated and glyco-oxidated species of both alpha and beta globin was demonstrated. Values for these showed a good linear relationship with HbA1c values and the mean daily plasma glucose values for the 6 weeks preceding the investigation. Trends differed according to whether patients had chronic complications or not, differences being seen in the slopes of the plots relating HbA1c to the abundance of glycated and glyco-oxidated species. CONCLUSIONS/ INTERPRETATION: The data obtained are consistent with the concept that individuals have a different individual proclivity for oxidation and/or that different oxidation kinetics are related to behavioural and environmental factors. Our data are thus relevant to the analysis of phenotype differences in diabeticpatients.
Authors: David E Goldstein; Randie R Little; Rodney A Lorenz; John I Malone; David M Nathan; Charles M Peterson Journal: Diabetes Care Date: 2003-01 Impact factor: 19.112
Authors: Curt L Rohlfing; Hsiao-Mei Wiedmeyer; Randie R Little; Jack D England; Alethea Tennill; David E Goldstein Journal: Diabetes Care Date: 2002-02 Impact factor: 19.112
Authors: A Lapolla; D Fedele; M Plebani; M Garbeglio; R Seraglia; M D'Alpaos; C N Aricò; P Traldi Journal: Rapid Commun Mass Spectrom Date: 1999 Impact factor: 2.419
Authors: A Lapolla; D Fedele; M Plebani; R Aronica; M Garbeglio; R Seraglia; M D'Alpaos; P Traldi Journal: Clin Chem Date: 1999-02 Impact factor: 8.327
Authors: Robert M Cohen; Robert S Franco; Paramjit K Khera; Eric P Smith; Christopher J Lindsell; Peter J Ciraolo; Mary B Palascak; Clinton H Joiner Journal: Blood Date: 2008-08-11 Impact factor: 22.113
Authors: Annunziata Lapolla; Eugenio Ragazzi; Barbara Andretta; Domenico Fedele; Michela Tubaro; Roberta Seraglia; Laura Molin; Pietro Traldi Journal: J Am Soc Mass Spectrom Date: 2007-02-22 Impact factor: 3.109