BACKGROUND: Many plants contain significant amounts of 4-coumaric acid (4CA), a compound with antioxidant properties in vitro and in vivo. The aim of this study was to assess the effects of 4CA pretreatment on DNA oxidative stress induced by intestinal inflammation in rodents. METHODS: 4CA (50 mg/kg) was administered to rats for 14 days mixed in the diet. Colitis was induced on days 13 and 14 by administering 6% (w/v) dextran sodium sulphate (DSS) in the drinking water. RESULTS: In the colon mucosa, DSS treatment increased myeloperoxidase activity (P < 0.05), oxidative DNA damage (P < 0.01), and cyclooxygenase-2 (COX-2) expression (P < 0.01) and reduced superoxide dismutase-2 (SOD-2) expression (P < 0.05). It was found that treatment with 4CA prior to DSS-induced inflammation reduced oxidative DNA damage (P < 0.01), COX-2 over-expression (P < 0.01) and restored SOD-2 gene expression to control levels. Similar effects were observed with nimesulide administered p.o. (5 mg/kg, 1 day before and during DSS treatment). PGE levels in plasma and colon mucosa were increased by DSS treatment and this effect was inhibited by pretreatment with 4-CA (P < 0.01). CONCLUSIONS: Mild acute intestinal inflammation induced by DSS can be inhibited by 4-CA and this action is associated with the suppression of COX-2 expression and activity.
BACKGROUND: Many plants contain significant amounts of 4-coumaric acid (4CA), a compound with antioxidant properties in vitro and in vivo. The aim of this study was to assess the effects of 4CA pretreatment on DNA oxidative stress induced by intestinal inflammation in rodents. METHODS: 4CA (50 mg/kg) was administered to rats for 14 days mixed in the diet. Colitis was induced on days 13 and 14 by administering 6% (w/v) dextran sodium sulphate (DSS) in the drinking water. RESULTS: In the colon mucosa, DSS treatment increased myeloperoxidase activity (P < 0.05), oxidative DNA damage (P < 0.01), and cyclooxygenase-2 (COX-2) expression (P < 0.01) and reduced superoxide dismutase-2 (SOD-2) expression (P < 0.05). It was found that treatment with 4CA prior to DSS-induced inflammation reduced oxidative DNA damage (P < 0.01), COX-2 over-expression (P < 0.01) and restored SOD-2 gene expression to control levels. Similar effects were observed with nimesulide administered p.o. (5 mg/kg, 1 day before and during DSS treatment). PGE levels in plasma and colon mucosa were increased by DSS treatment and this effect was inhibited by pretreatment with 4-CA (P < 0.01). CONCLUSIONS: Mild acute intestinal inflammation induced by DSS can be inhibited by 4-CA and this action is associated with the suppression of COX-2 expression and activity.
Authors: Mustafa Guven; Adem Bozkurt Aras; Tarik Akman; Halil Murat Sen; Adile Ozkan; Osman Salis; Ibrahim Sehitoglu; Yildiray Kalkan; Coskun Silan; Mustafa Deniz; Murat Cosar Journal: Iran J Basic Med Sci Date: 2015-04 Impact factor: 2.699
Authors: Elisabetta Bigagli; Mario D'Ambrosio; Lorenzo Cinci; Alberto Niccolai; Natascia Biondi; Liliana Rodolfi; Luana Beatriz Dos Santos Nascimiento; Mario R Tredici; Cristina Luceri Journal: Mar Drugs Date: 2021-06-11 Impact factor: 5.118
Authors: Sohair M M Ragab; Sary Kh Abd Elghaffar; Tarek H El-Metwally; Gamal Badr; Mohamed H Mahmoud; Hossam M Omar Journal: Lipids Health Dis Date: 2015-07-31 Impact factor: 3.876