Culture techniques for human keratinocytes.

The two main approaches developed for in vitro culture of human keratinocytes are reviewed and discussed. The older technique is based on the use of a serum-containing medium and of a feeder-layer of lethally irradiated mouse fibroblasts; the second relies upon serum-free media, in the absence of a feeder-layer. While the former technique is most widely used for the preparation of epithelial sheets for grafting, the latter is best suited for studies on the biological properties of keratinocytes.